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Literature summary for 1.4.1.4 extracted from

  • Kobayashi, T.; Higuchi, S.; Kimura, K.; Kudo, T.; Horikoshi, K.
    Properties of glutamate dehydrogenase and its involvement in alanine production in a hyperthermophilic archaeon, Thermococcus profundus (1995), J. Biochem., 118, 587-592.
    View publication on PubMed

Inhibitors

Inhibitors Comment Organism Structure
Hg2+ at 0.1 mM, complete activity loss Thermococcus profundus

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
43000
-
alpha6, 6 * 43000, SDS-PAGE Thermococcus profundus
263000
-
-
Thermococcus profundus

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
L-glutamate + NADP+ + H2O Thermococcus profundus
-
2-oxoglutarate + NADPH + NH3
-
r

Organism

Organism UniProt Comment Textmining
Thermococcus profundus
-
-
-

Purification (Commentary)

Purification (Comment) Organism
-
Thermococcus profundus

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
L-glutamate + NADP+ + H2O
-
Thermococcus profundus 2-oxoglutarate + NADPH + NH3
-
r

Subunits

Subunits Comment Organism
homohexamer alpha6, 6 * 43000, SDS-PAGE Thermococcus profundus

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
85
-
-
Thermococcus profundus

Temperature Stability [°C]

Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
80
-
50% activity after 4 h Thermococcus profundus
90
-
50% activity after 1 h Thermococcus profundus

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
7
-
oxidative deamination Thermococcus profundus
7.2
-
reductive amination Thermococcus profundus

pH Range

pH Minimum pH Maximum Comment Organism
4 10 fully active after 60 min Thermococcus profundus

Cofactor

Cofactor Comment Organism Structure
NADP+
-
Thermococcus profundus
NADPH
-
Thermococcus profundus