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Literature summary for 1.4.1.4 extracted from

  • Ohshima, T.; Nishida, N.
    Purification and properties of extremely thermostable glutamate dehydrogenase from two hyperthermophilic archaebacteria, Pyrococcus woesei and Pyrococcus furiosus (1993), Biosci. Biotechnol. Biochem., 57, 945-951.
    View publication on PubMed
Search for more literature for 1.4.1.4

Activating Compound

Activating Compound Comment Organism Structure
KCl at concentrations below 1 mM Pyrococcus woesei
KCl at concentrations below 2 mM Pyrococcus furiosus

Inhibitors

Inhibitors Comment Organism Structure
AlCl3 at 1 mM, 30% inhibition Pyrococcus furiosus
AlCl3 at 1 mM, 21% inhibition Pyrococcus woesei
HgCl2 at 1 mM, 64% inhibition Pyrococcus furiosus
HgCl2 at 1 mM, 45% inhibition Pyrococcus woesei
MnCl2 at 1 mM, 63% inhibition Pyrococcus furiosus
MnCl2 at 1 mM, 20% inhibition Pyrococcus woesei
Pb(CH3COO)2 at 1 mM, 59% inhibition Pyrococcus furiosus
Pb(CH3COO)2 at 1 mM, 64% inhibition Pyrococcus woesei
pyridoxal 5'-phosphate at 1 mM, 35% inhibition Pyrococcus furiosus
pyridoxal 5'-phosphate at 1 mM, 45% inhibition Pyrococcus woesei
Urea inactivation with 2 mM urea Pyrococcus furiosus
Urea fully active at 20°C in 8 mM urea Pyrococcus woesei

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
47000
-
 alpha6, 6 * 47000, SDS-PAGE Pyrococcus furiosus
47000
-
 alpha6, 6 * 47000, SDS-PAGE Pyrococcus woesei
300000
-
 gel filtration Pyrococcus furiosus
300000
-
 gel filtration Pyrococcus woesei

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
L-glutamate + NADP+ + H2O Pyrococcus furiosus
-
 2-oxoglutarate + NADPH + NH3
-
 r
L-glutamate + NADP+ + H2O Pyrococcus woesei
-
 2-oxoglutarate + NADPH + NH3
-
 r

Organism

Organism UniProt Comment Textmining
Pyrococcus furiosus
-
 strain DSM 3638
-
Pyrococcus woesei
-
 strain DSM 3773
-

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
10.3
-
 oxidative deamination at 50°C and pH 8.2 Pyrococcus furiosus
24.3
-
 oxidative deamination at 50°C and pH 8.2 Pyrococcus woesei

Storage Stability

Storage Stability Organism
4°C, 10 mM potassium phosphate buffer, pH 7.2, 0.1 mM EDTA, 0.1 mM DTT, 5 months, no activity loss Pyrococcus furiosus
4°C, 10 mM potassium phosphate buffer, pH 7.2, 0.1 mM EDTA, 0.1 mM DTT, 5 months, no activity loss Pyrococcus woesei

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
L-glutamate + NADP+ + H2O
-
 Pyrococcus furiosus 2-oxoglutarate + NADPH + NH3
-
 r
L-glutamate + NADP+ + H2O
-
 Pyrococcus woesei 2-oxoglutarate + NADPH + NH3
-
 r

Subunits

Subunits Comment Organism
homohexamer alpha6, 6 * 47000, SDS-PAGE Pyrococcus furiosus
homohexamer alpha6, 6 * 47000, SDS-PAGE Pyrococcus woesei

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
100
-
 oxidative deamination Pyrococcus furiosus
100
-
 oxidative deamination Pyrococcus woesei

Temperature Stability [°C]

Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
105
-
 full activity after 10 min, 50% activity after 90 min Pyrococcus furiosus
105
-
 full activity after 10 min, 50% activity after 90 min Pyrococcus woesei
115
-
 inactivation after 10 min Pyrococcus furiosus
115
-
 inactivation after 10 min Pyrococcus woesei

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
7.4
-
 reductive amination at 50°C Pyrococcus furiosus
7.4
-
 reductive amination at 50°C Pyrococcus woesei
8.2
-
 oxidative deamination at 50°C Pyrococcus furiosus
8.2
-
 oxidative deamination at 50°C Pyrococcus woesei

pH Range

pH Minimum pH Maximum Comment Organism
4 10
-
 Pyrococcus furiosus
4 10
-
 Pyrococcus woesei

Cofactor

Cofactor Comment Organism Structure
NADP+
-
 Pyrococcus furiosus
NADP+
-
 Pyrococcus woesei
NADPH
-
 Pyrococcus furiosus
NADPH
-
 Pyrococcus woesei