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Literature summary for 1.4.1.4 extracted from

  • Botton, B.; Msatef, Y.
    Purification and properties of NADP-dependent glutamate dehydrogenase from Sphaerostilbe repens (1983), Physiol. Plant., 59, 438-444.
No PubMed abstract available

General Stability

General Stability Organism
2-mercaptoethanol and dithiothreitol stabilizes Corallomycetella repens

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.043
-
NADP+ oxidative deamination Corallomycetella repens
0.049
-
NADPH reductive amination Corallomycetella repens
0.64
-
2-oxoglutarate reductive amination Corallomycetella repens
2.6
-
NH4+ reductive amination, biphasic kinetics Corallomycetella repens
5.5
-
L-glutamate oxidative deamination Corallomycetella repens
21.2
-
NH4+ reductive amination Corallomycetella repens
21.2
-
NH4+ biphasic kinetics Corallomycetella repens

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
48000
-
alpha6, 6 * 48000, SDS-PAGE Corallomycetella repens
280000
-
non-denaturating PAGE Corallomycetella repens

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
L-glutamate + NADP+ + H2O Corallomycetella repens
-
2-oxoglutarate + NADPH + NH3
-
r

Organism

Organism UniProt Comment Textmining
Corallomycetella repens
-
-
-

Storage Stability

Storage Stability Organism
4°C, ten days and 25°C, 15 h, both 100% activity Corallomycetella repens

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2-oxoglutarate + NH4+ + NADPH
-
Corallomycetella repens L-glutamate + NADP+
-
?
L-glutamate + NADP+ + H2O
-
Corallomycetella repens 2-oxoglutarate + NADPH + NH3
-
r
L-glutamate + NADP+ + H2O
-
Corallomycetella repens 2-oxoglutarate + NADPH + NH3 biphasic kinetic behavior for ammonia r

Subunits

Subunits Comment Organism
homohexamer alpha6, 6 * 48000, SDS-PAGE Corallomycetella repens

Temperature Stability [°C]

Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
20
-
stable for 15 h Corallomycetella repens

Turnover Number [1/s]

Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
1100
-
NADPH
-
Corallomycetella repens

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
6.9
-
reductive amination Corallomycetella repens
8.4
-
oxidative deamination Corallomycetella repens

Cofactor

Cofactor Comment Organism Structure
NADP+
-
Corallomycetella repens
NADPH
-
Corallomycetella repens