Any feedback?
Please rate this page
(literature.php)
(0/150)

BRENDA support

Literature summary for 1.4.1.4 extracted from

  • Kim, Y.J.; Yoshizawa, M.; Takenaka, S.; Murakami, S.; Aoki, K.
    Ammonia assimilation in Klebsiella pneumoniae F-5-2 that can utilize ammonium and nitrate ions simultaneously: purification and characterization of glutamate dehydrogenase and glutamine synthetase (2002), J. Biosci. Bioeng., 93, 584-588.
    View publication on PubMed
Search for more literature for 1.4.1.4

Inhibitors

Inhibitors Comment Organism Structure
AgNO3 1 mM, 30°C, complete loss of aminating activity Klebsiella pneumoniae
AlCl3 1 mM, 30°C, 23% loss of aminating activity Klebsiella pneumoniae
BaCl2 1 mM, 30°C, 15% loss of aminating activity Klebsiella pneumoniae
CaCl2 1 mM, 30°C, 11% loss of aminating activity Klebsiella pneumoniae
CH2ICOOH 1 mM, 30°C, 15% loss of aminating activity Klebsiella pneumoniae
FeSO3(NH4)2SO4 1 mM, 30°C, 14% loss of aminating activity Klebsiella pneumoniae
HgCl2 1 mM, 30°C, 48% loss of aminating activity Klebsiella pneumoniae

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
52000
-
 6 * 52000, SDS-PAGE Klebsiella pneumoniae
300000
-
 gel filtration Klebsiella pneumoniae

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
2-oxoglutarate + NADPH + NH3 Klebsiella pneumoniae ammonia-assimilating enzyme L-glutamate + NADP+ + H2O
-
 r
2-oxoglutarate + NADPH + NH3 Klebsiella pneumoniae F-5-2 ammonia-assimilating enzyme L-glutamate + NADP+ + H2O
-
 r

Organism

Organism UniProt Comment Textmining
Klebsiella pneumoniae
-
-
-
Klebsiella pneumoniae F-5-2
-
-
-

Purification (Commentary)

Purification (Comment) Organism
-
 Klebsiella pneumoniae

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
190
-
-
 Klebsiella pneumoniae

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2-oxoadipate + NADPH + NH3 6.3% of the activity with 2-oxoglutarate Klebsiella pneumoniae L-2-aminoadipate + NADP+ + H2O
-
 ?
2-oxoadipate + NADPH + NH3 6.3% of the activity with 2-oxoglutarate Klebsiella pneumoniae F-5-2 L-2-aminoadipate + NADP+ + H2O
-
 ?
2-oxobutyrate + NADPH + NH3 12% of the activity with 2-oxoglutarate Klebsiella pneumoniae L-2-aminobutyrate + NADP+ + H2O
-
 ?
2-oxobutyrate + NADPH + NH3 12% of the activity with 2-oxoglutarate Klebsiella pneumoniae F-5-2 L-2-aminobutyrate + NADP+ + H2O
-
 ?
2-oxoglutarate + NADPH + NH3
-
 Klebsiella pneumoniae L-glutamate + NADP+ + H2O
-
 r
2-oxoglutarate + NADPH + NH3 ammonia-assimilating enzyme Klebsiella pneumoniae L-glutamate + NADP+ + H2O
-
 r
2-oxoglutarate + NADPH + NH3
-
 Klebsiella pneumoniae F-5-2 L-glutamate + NADP+ + H2O
-
 r
2-oxoglutarate + NADPH + NH3 ammonia-assimilating enzyme Klebsiella pneumoniae F-5-2 L-glutamate + NADP+ + H2O
-
 r
2-oxovalerate + NADPH + NH3 15% of the activity with 2-oxoglutarate Klebsiella pneumoniae L-2-aminovalerate + NADP+ + H2O
-
 ?
additional information no activity with 2-oxoisovalerate and pyruvate Klebsiella pneumoniae ?
-
 ?
additional information no activity with 2-oxoisovalerate and pyruvate Klebsiella pneumoniae F-5-2 ?
-
 ?

Subunits

Subunits Comment Organism
hexamer 6 * 52000, SDS-PAGE Klebsiella pneumoniae

Temperature Stability [°C]

Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
50
-
 pH 8.0, 10 min, maintains more than 90% activity Klebsiella pneumoniae

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
8
-
 aminating activity Klebsiella pneumoniae
9 10 deaminating activity Klebsiella pneumoniae

pH Stability

pH Stability pH Stability Maximum Comment Organism
5.5 11.5 stable Klebsiella pneumoniae

Cofactor

Cofactor Comment Organism Structure
NADP+
-
 Klebsiella pneumoniae
NADPH
-
 Klebsiella pneumoniae