Inhibitors | Comment | Organism | Structure |
---|---|---|---|
AgNO3 | 1 mM, 30°C, complete loss of aminating activity | Klebsiella pneumoniae | |
AlCl3 | 1 mM, 30°C, 23% loss of aminating activity | Klebsiella pneumoniae | |
BaCl2 | 1 mM, 30°C, 15% loss of aminating activity | Klebsiella pneumoniae | |
CaCl2 | 1 mM, 30°C, 11% loss of aminating activity | Klebsiella pneumoniae | |
CH2ICOOH | 1 mM, 30°C, 15% loss of aminating activity | Klebsiella pneumoniae | |
FeSO3(NH4)2SO4 | 1 mM, 30°C, 14% loss of aminating activity | Klebsiella pneumoniae | |
HgCl2 | 1 mM, 30°C, 48% loss of aminating activity | Klebsiella pneumoniae |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
52000 | - |
6 * 52000, SDS-PAGE | Klebsiella pneumoniae |
300000 | - |
gel filtration | Klebsiella pneumoniae |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
2-oxoglutarate + NADPH + NH3 | Klebsiella pneumoniae | ammonia-assimilating enzyme | L-glutamate + NADP+ + H2O | - |
r | |
2-oxoglutarate + NADPH + NH3 | Klebsiella pneumoniae F-5-2 | ammonia-assimilating enzyme | L-glutamate + NADP+ + H2O | - |
r |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Klebsiella pneumoniae | - |
- |
- |
Klebsiella pneumoniae F-5-2 | - |
- |
- |
Purification (Comment) | Organism |
---|---|
- |
Klebsiella pneumoniae |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
190 | - |
- |
Klebsiella pneumoniae |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
2-oxoadipate + NADPH + NH3 | 6.3% of the activity with 2-oxoglutarate | Klebsiella pneumoniae | L-2-aminoadipate + NADP+ + H2O | - |
? | |
2-oxoadipate + NADPH + NH3 | 6.3% of the activity with 2-oxoglutarate | Klebsiella pneumoniae F-5-2 | L-2-aminoadipate + NADP+ + H2O | - |
? | |
2-oxobutyrate + NADPH + NH3 | 12% of the activity with 2-oxoglutarate | Klebsiella pneumoniae | L-2-aminobutyrate + NADP+ + H2O | - |
? | |
2-oxobutyrate + NADPH + NH3 | 12% of the activity with 2-oxoglutarate | Klebsiella pneumoniae F-5-2 | L-2-aminobutyrate + NADP+ + H2O | - |
? | |
2-oxoglutarate + NADPH + NH3 | - |
Klebsiella pneumoniae | L-glutamate + NADP+ + H2O | - |
r | |
2-oxoglutarate + NADPH + NH3 | ammonia-assimilating enzyme | Klebsiella pneumoniae | L-glutamate + NADP+ + H2O | - |
r | |
2-oxoglutarate + NADPH + NH3 | - |
Klebsiella pneumoniae F-5-2 | L-glutamate + NADP+ + H2O | - |
r | |
2-oxoglutarate + NADPH + NH3 | ammonia-assimilating enzyme | Klebsiella pneumoniae F-5-2 | L-glutamate + NADP+ + H2O | - |
r | |
2-oxovalerate + NADPH + NH3 | 15% of the activity with 2-oxoglutarate | Klebsiella pneumoniae | L-2-aminovalerate + NADP+ + H2O | - |
? | |
additional information | no activity with 2-oxoisovalerate and pyruvate | Klebsiella pneumoniae | ? | - |
? | |
additional information | no activity with 2-oxoisovalerate and pyruvate | Klebsiella pneumoniae F-5-2 | ? | - |
? |
Subunits | Comment | Organism |
---|---|---|
hexamer | 6 * 52000, SDS-PAGE | Klebsiella pneumoniae |
Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
---|---|---|---|
50 | - |
pH 8.0, 10 min, maintains more than 90% activity | Klebsiella pneumoniae |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
8 | - |
aminating activity | Klebsiella pneumoniae |
9 | 10 | deaminating activity | Klebsiella pneumoniae |
pH Stability | pH Stability Maximum | Comment | Organism |
---|---|---|---|
5.5 | 11.5 | stable | Klebsiella pneumoniae |
Cofactor | Comment | Organism | Structure |
---|---|---|---|
NADP+ | - |
Klebsiella pneumoniae | |
NADPH | - |
Klebsiella pneumoniae |