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Literature summary for 1.4.1.4 extracted from
- Extremely thermostable glutamate dehydrogenase from the hyperthermophilic archaebacterium Pyrococcus furiosus (1991), Eur. J. Biochem., 202, 1189-1196.
General Stability
| General Stability | Organism |
|---|---|
| the purified enzyme can withstand at least three freezing (-20°C) and thawing (25°C) cycles without significant effects on activity the enzyme can be lyophilzed and stored for several months without loss of activity | Pyrococcus furiosus |
Inhibitors
| Inhibitors | Comment | Organism | Structure |
|---|---|---|---|
| 2-oxoglutarate | substrate inhibition above 2.0 mM | Pyrococcus furiosus |  |
| L-glutamate | substrate inhibition above 15 mM | Pyrococcus furiosus | |
| additional information | no effect: EDTA, o-phenanthroline, sodium azide, 8-hydroxyquinoline in the concentration raneg of 1-10 mM | Pyrococcus furiosus |
KM Value [mM]
| KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 0.012 | - |
NADPH | pH 7.6, 60°C | Pyrococcus furiosus | |
| 0.02 | - |
NADP+ | pH 7.6, 60°C | Pyrococcus furiosus | |
| 0.36 | - |
NADH | pH 7.6, 50°C | Pyrococcus furiosus | |
| 0.6 | - |
L-glutamate | pH 7.6, 60°C | Pyrococcus furiosus | |
| 6 | - |
2-oxoglutarate | pH 7.6, 60°C | Pyrococcus furiosus | |
Molecular Weight [Da]
| Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|
| 48000 | - |
x * 48000, SDS-PAGE | Pyrococcus furiosus |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Pyrococcus furiosus | - |
- |
- |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
- |
Pyrococcus furiosus |
Storage Stability
| Storage Stability | Organism |
|---|---|
| 25°C, 20 mM potassium phosphate, pH 6.5, containing 7 mM 2-mercaptoethanol and 10% glycerol, stable for several weeks | Pyrococcus furiosus |
| 4°C, 20 mM potassium phosphate, pH 6.5, containing 7 mM 2-mercaptoethanol and 10% glycerol, stable for several weeks | Pyrococcus furiosus |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| 2-aminopentanoate + H2O + NADP+ | activity with 2-oxovalerate amd 2-aminopentanoate is 25% and 17% compared to the activity with 2-oxoglutarate and L-glutamate | Pyrococcus furiosus | 2-oxopentanoate + NH3 + NADPH + H+ | - |
r | |
| 2-oxoglutarate + NH3 + NADH + H+ | weak activity | Pyrococcus furiosus | L-glutamate + H2O + NAD+ | - |
? | |
| L-glutamate + H2O + NADP+ | - |
Pyrococcus furiosus | 2-oxoglutarate + NH3 + NADPH + H+ | - |
r | |
Subunits
| Subunits | Comment | Organism |
|---|---|---|
| ? | x * 48000, SDS-PAGE | Pyrococcus furiosus |
Temperature Optimum [°C]
| Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 60 | - |
assay at | Pyrococcus furiosus |
| 75 | - |
reductive amination | Pyrococcus furiosus |
| 80 | - |
oxidation of glutamate | Pyrococcus furiosus |
Temperature Stability [°C]
| Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 90 | - |
20 h, stable | Pyrococcus furiosus |
| 100 | - |
half-life: 12 h | Pyrococcus furiosus |
| 110 | - |
half-life: 25 min | Pyrococcus furiosus |
pH Optimum
| pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|
| 7.6 | - |
assay at | Pyrococcus furiosus |
| 9 | - |
- |
Pyrococcus furiosus |
Cofactor
| Cofactor | Comment | Organism | Structure |
|---|---|---|---|
| NAD+ | low activity | Pyrococcus furiosus | |
| NADP+ | - |
Pyrococcus furiosus | |
| NADPH | - |
Pyrococcus furiosus | |
pI Value
| Organism | Comment | pI Value Maximum | pI Value |
|---|---|---|---|
| Pyrococcus furiosus | isoelectrofocusing | - |
4.5 |
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Release 2023.1 (January 2023)
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