Organic Solvent | Comment | Organism |
---|---|---|
guanidine-HCl | the denaturation process is accompanied by an exposure to the solvent of the tryptophan residues, as manifested by the red shift of the emission maximum in both cases. The unfolding of Haloferax mediterranei glutamate dehydrogenase is a gradual process, which is accompanied by a loss in enzyme activity. Fluorescence quenching by external quenchers, KI and acrylamide, has also been carried out. The tryptophan residues in the protein are more exposed to the solvent in Haloferax mediterranei than in bovine glutamate dehydrogenase. The total amount of tryptophan residues is nearly the same for both enzymes | Bos taurus |
guanidine-HCl | the denaturation process is accompanied by an exposure to the solvent of the tryptophan residues, as manifested by the red shift of the emission maximum in both cases. The unfolding of Haloferax mediterranei glutamate dehydrogenase is a gradual process, which is accompanied by a loss in enzyme activity. Fluorescence quenching by external quenchers, KI and acrylamide, has also been carried out. The tryptophan residues in the protein are more exposed to the solvent in Haloferax mediterranei than in bovine glutamate dehydrogenase. The total amount of tryptophan residues is nearly the same for both enzymes | Haloferax mediterranei |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Bos taurus | - |
- |
- |
Haloferax mediterranei | - |
- |
- |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
L-glutamate + H2O + NADP+ | - |
Bos taurus | 2-oxoglutarate + NH3 + NADPH + H+ | - |
? | |
L-glutamate + H2O + NADP+ | - |
Haloferax mediterranei | 2-oxoglutarate + NH3 + NADPH + H+ | - |
? |
Subunits | Comment | Organism |
---|---|---|
homohexamer | - |
Bos taurus |
homohexamer | - |
Haloferax mediterranei |
Synonyms | Comment | Organism |
---|---|---|
NADP-glutamate dehydrogenase | - |
Bos taurus |
NADP-glutamate dehydrogenase | - |
Haloferax mediterranei |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
25 | - |
assay at | Bos taurus |
25 | - |
assay at | Haloferax mediterranei |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
8 | - |
assay at | Bos taurus |
8 | - |
assay at | Haloferax mediterranei |