Inhibitors | Comment | Organism | Structure |
---|---|---|---|
Zn2+ | - |
Bacillus subtilis |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.16 | - |
NAD+ | (+ L-isoleucine) | Bacillus subtilis | |
2.2 | - |
L-isoleucine | - |
Bacillus subtilis | |
25 | - |
L-valine | - |
Bacillus subtilis | |
33 | - |
NAD+ | (+ L-valine) | Bacillus subtilis |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
290000 | - |
gel filtration | Bacillus subtilis |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
L-Amino acid + H2O + NAD+ | Bacillus subtilis | - |
? | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Bacillus subtilis | - |
- |
- |
Purification (Comment) | Organism |
---|---|
partial | Bacillus subtilis |
Source Tissue | Comment | Organism | Textmining |
---|---|---|---|
spore | resting | Bacillus subtilis | - |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
L-Amino acid + H2O + NAD+ | aliphatic amino acids, e.g. L-valine, L-leucine, L-isoleucine | Bacillus subtilis | 2-Oxo acid + NH3 + NADH | - |
? | |
L-Amino acid + H2O + NAD+ | - |
Bacillus subtilis | ? | - |
? | |
L-isoleucine + H2O + NAD+ | - |
Bacillus subtilis | 3-methyl-2-oxopentanoate + NH3 + NADH + H+ | - |
r | |
L-valine + H2O + NAD+ | - |
Bacillus subtilis | 3-methyl-2-oxobutanoate + NH3 + NADH + H+ | - |
r |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
37 | - |
assay at | Bacillus subtilis |
Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
---|---|---|---|
20 | - |
10 min, 25-35% loss of activity | Bacillus subtilis |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
10.7 | - |
L-valine, L-isoleucine | Bacillus subtilis |
Cofactor | Comment | Organism | Structure |
---|---|---|---|
NAD+ | cannot be replaced by NADP+ | Bacillus subtilis |