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Literature summary for 1.4.1.9 extracted from

  • Oka, M.; Yang, Y.S.; Nagata, S.; Esaki, N.; Tanaka, H.; Soda, K.
    Overproduction of thermostable leucine dehydrogenase of Bacillus stearothermophilus and its one-step purification from recombinant cells of Escherichia coli (1989), Biotechnol. Appl. Biochem., 11, 307-311.
    View publication on PubMed

Cloned(Commentary)

Cloned (Comment) Organism
expression in Escherichia coli Geobacillus stearothermophilus

Organism

Organism UniProt Comment Textmining
Geobacillus stearothermophilus
-
-
-

Purification (Commentary)

Purification (Comment) Organism
one-step purification of recombinant enzyme Geobacillus stearothermophilus

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
120
-
-
Geobacillus stearothermophilus

Storage Stability

Storage Stability Organism
4°C, buffer containing 0.02% sodium azide, stable for more than 1 year Geobacillus stearothermophilus

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
L-Leu + H2O + NAD+
-
Geobacillus stearothermophilus 4-methyl-2-oxopentanoate + NH3 + NADH
-
r

Temperature Stability [°C]

Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
55
-
pH 6.0-11.0, 10 min, stable Geobacillus stearothermophilus
70
-
30 min, retains full activity Geobacillus stearothermophilus

pH Stability

pH Stability pH Stability Maximum Comment Organism
6 11 55°C, 10 min, stable Geobacillus stearothermophilus

Cofactor

Cofactor Comment Organism Structure
NAD+
-
Geobacillus stearothermophilus
NADH
-
Geobacillus stearothermophilus