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Literature summary for 1.4.1.9 extracted from

  • Livesey, G.; Lund, P.
    Determination of branched-chain amino and keto acids with leucine dehydrogenase (1988), Methods Enzymol., 166, 3-10.
    View publication on PubMed

Application

Application Comment Organism
analysis cheap and rapid determination of branched-chain amino and oxo acids Bacillus subtilis
analysis cheap and rapid determination of branched-chain amino and oxo acids Lysinibacillus sphaericus

Organism

Organism UniProt Comment Textmining
Bacillus subtilis
-
-
-
Lysinibacillus sphaericus
-
-
-

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
L-Ile + H2O + NAD+
-
Bacillus subtilis 3-methyl-2-oxopentanoate + NH3 + NADH
-
r
L-Ile + H2O + NAD+
-
Lysinibacillus sphaericus 3-methyl-2-oxopentanoate + NH3 + NADH
-
r
L-Leu + H2O + NAD+
-
Bacillus subtilis 4-methyl-2-oxopentanoate + NH3 + NADH
-
r
L-Leu + H2O + NAD+
-
Lysinibacillus sphaericus 4-methyl-2-oxopentanoate + NH3 + NADH
-
r
L-Val + H2O + NAD+
-
Bacillus subtilis 3-methyl-2-oxobutanoate + NH3 + NADH
-
?
L-Val + H2O + NAD+
-
Lysinibacillus sphaericus 3-methyl-2-oxobutanoate + NH3 + NADH
-
?

Cofactor

Cofactor Comment Organism Structure
NAD+
-
Bacillus subtilis
NAD+
-
Lysinibacillus sphaericus
NADH
-
Bacillus subtilis
NADH
-
Lysinibacillus sphaericus