Any feedback?
Please rate this page
(literature.php)
(0/150)

BRENDA support

Literature summary for 1.4.1.9 extracted from

  • Zhao, Y.; Wakamatsu, T.; Doi, K.; Sakuraba, H.; Ohshima, T.
    A psychrophilic leucine dehydrogenase from Sporosarcina psychrophila: purification, characterization, gene sequencing and crystal structure analysis (2012), J. Mol. Catal. B, 83, 65-72.
No PubMed abstract available
Search for more literature for 1.4.1.9

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.015
-
 NAD+ pH 10.5, 20°C Sporosarcina psychrophila
0.65
-
 L-leucine pH 10.5, 20°C Sporosarcina psychrophila

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
43000
-
 8 * 43000, SDS-PAGE Sporosarcina psychrophila
340000
-
 gel filtration Sporosarcina psychrophila

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
L-leucine + H2O + NAD+ Sporosarcina psychrophila
-
 4-methyl-2-oxopentanoate + NH3 + NADH + H+
-
 ?
L-leucine + H2O + NAD+ Sporosarcina psychrophila DSM 3
-
 4-methyl-2-oxopentanoate + NH3 + NADH + H+
-
 ?

Organism

Organism UniProt Comment Textmining
Sporosarcina psychrophila
-
-
-
Sporosarcina psychrophila DSM 3
-
-
-

Purification (Commentary)

Purification (Comment) Organism
native enzyme 48fold by ammonium sulfate fractionation, anion exchange and hydrophobic interaction chromatography , and gel filtration to over 95% purity Sporosarcina psychrophila

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
0.074
-
 strain DSM 3, pH 10.5, 25°C Sporosarcina psychrophila
53.8
-
 purified enzyme, pH 10.5, 25°C Sporosarcina psychrophila

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
L-leucine + H2O + NAD+
-
 Sporosarcina psychrophila 4-methyl-2-oxopentanoate + NH3 + NADH + H+
-
 ?
L-leucine + H2O + NAD+
-
 Sporosarcina psychrophila DSM 3 4-methyl-2-oxopentanoate + NH3 + NADH + H+
-
 ?

Subunits

Subunits Comment Organism
homooctamer 8 * 43000, SDS-PAGE Sporosarcina psychrophila

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
50
-
-
 Sporosarcina psychrophila

Temperature Range [°C]

Temperature Minimum [°C] Temperature Maximum [°C] Comment Organism
-
 50 highest activity at 50°C, 10% of maximal activity at 0°C Sporosarcina psychrophila

Temperature Stability [°C]

Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
25
-
 purified enzyme, fully stable at pH 5.0-11.0 Sporosarcina psychrophila
40
-
 purified enzyme, 40 min, fully stable Sporosarcina psychrophila
50
-
 purified enzyme, half-life is 30 min Sporosarcina psychrophila
55
-
 purified enzyme, half-life is 10 min Sporosarcina psychrophila

Turnover Number [1/s]

Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
10
-
 L-leucine pH 10.5, 20°C Sporosarcina psychrophila

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
9
-
 amination of 4-methyl-2-oxopentanoate Sporosarcina psychrophila
11
-
 deamination of l-leucine Sporosarcina psychrophila

pH Stability

pH Stability pH Stability Maximum Comment Organism
5 11 purified enzyme, fully stable at 25°C Sporosarcina psychrophila

Cofactor

Cofactor Comment Organism Structure
NAD+
-
 Sporosarcina psychrophila
NADH
-
 Sporosarcina psychrophila