Inhibitors | Comment | Organism | Structure |
---|---|---|---|
2,4-Diaminopentanoate | - |
Acetoanaerobium sticklandii | |
2,5-Diaminohexanoate | - |
Acetoanaerobium sticklandii | |
5-amino-3-oxohexanoate | competitive inhibition | Acetoanaerobium sticklandii | |
ADP | about half as effective as ATP | Acetoanaerobium sticklandii | |
AMP | 32% as effective as ATP | Acetoanaerobium sticklandii | |
arsenate | addition of any of the neutral salts causes a parabolic inhibition. A direct comparison of arsenate and chloride ion shows that arsenate is not as inhibitory even though it has a higher ionic strength. Arsenate interacts with the enzyme differently from chloride and bromide. Sulfate, which is a large ion like arsenate, is as inhibitory as chloride (at equivalent ionic strength) with NAD+ as coenzyme, but it is much less effective than chloride with NADP+ as coenzyme | Acetoanaerobium sticklandii | |
ATP | competitive with respect to L-erythro-3,5-diaminohexanoate and NAD+ | Acetoanaerobium sticklandii | |
Br- | addition of any of the neutral salts causes a parabolic inhibition. A direct comparison of arsenate and chloride ion shows that arsenate is not as inhibitory even though it has a higher ionic strength. Arsenate interacts with the enzyme differently from chloride and bromide. Sulfate, which is a large ion like arsenate, is as inhibitory as chloride (at equivalent ionic strength) with NAD+ as coenzyme, but it is much less effective than chloride with NADP+ as coenzyme | Acetoanaerobium sticklandii | |
Ca2+ | 0.5 mM, 22% inhibition at pH 7.8, cofactor NADP+, activation at pH 8.9 | Acetoanaerobium sticklandii | |
Cl- | addition of any of the neutral salts causes a parabolic inhibition. A direct comparison of arsenate and chloride ion shows that arsenate is not as inhibitory even though it has a higher ionic strength. Arsenate interacts with the enzyme differently from chloride and bromide. Sulfate, which is a large ion like arsenate, is as inhibitory as chloride (at equivalent ionic strength) with NAD+ as coenzyme, but it is much less effective than chloride with NADP+ as coenzyme | Acetoanaerobium sticklandii | |
Co2+ | 0.5 mM, 71% inhibition at pH 7.8, cofactor NADP+, activation at pH 8.9 | Acetoanaerobium sticklandii | |
CTP | about half as effective as ATP | Acetoanaerobium sticklandii | |
Cu2+ | 0.5 mM, 35% inhibition at pH 7.8, 30% inhibition at pH 8.9, cofactor NADP+ | Acetoanaerobium sticklandii | |
D-erythro-3,5-diaminohexanoate | - |
Acetoanaerobium sticklandii | |
D-Lysine | - |
Acetoanaerobium sticklandii | |
DL-beta-aminobutyrate | - |
Acetoanaerobium sticklandii | |
Fe2+ | 0.5 mM, 36% inhibition at pH 7.8, 9% inhibition at pH 8.9, cofactor NADP+ | Acetoanaerobium sticklandii | |
Fe3+ | 0.5 mM, 8% inhibition at pH 8.9, no effect on activity at pH 7.8 | Acetoanaerobium sticklandii | |
GTP | inhibits as effectively as ATP | Acetoanaerobium sticklandii | |
L-beta-Lysine | - |
Acetoanaerobium sticklandii | |
L-lysine | - |
Acetoanaerobium sticklandii | |
L-ornithine | - |
Acetoanaerobium sticklandii | |
Mg2+ | 0.5 mM, 63% inhibition at pH 7.8, cofactor NADP+, activation at pH 8.9 | Acetoanaerobium sticklandii | |
Mn2+ | 0.5 mM, 52% inhibition at pH 7.8, cofactor NADP+, activation at pH 8.9 | Acetoanaerobium sticklandii | |
additional information | the lactams of DL-erythro-3,5-diaminohexanoate and DL-threo-3,5-diaminohexanoatae and 2-methylpyrrolidone-5-carboxylic acid have no effect on activity. Acetate (10 mM), butyrate (10 mM), acetyl phosphate (2 mM), or acetyl-CoA (0.62 mM) have no effect on enzyme activity | Acetoanaerobium sticklandii | |
NADH | competitive inhibition | Acetoanaerobium sticklandii | |
NADPH | competitive inhibition | Acetoanaerobium sticklandii | |
NH4+ | 10 mM NH4Cl and higher, inhibits the reaction, probably due to the effect of chloride on the activity | Acetoanaerobium sticklandii | |
SO42- | addition of any of the neutral salts causes a parabolic inhibition. A direct comparison of arsenate and chloride ion shows that arsenate is not as inhibitory even though it has a higher ionic strength. Arsenate interacts with the enzyme differently from chloride and bromide. Sulfate, which is a large ion like arsenate, is as inhibitory as chloride (at equivalent ionic strength) with NAD+ as coenzyme, but it is much less effective than chloride with NADP+ as coenzyme | Acetoanaerobium sticklandii | |
UTP | 16% as effective as ATP | Acetoanaerobium sticklandii | |
Zn2+ | 0.5 mM, 49% inhibition at pH 7.8, cofactor NADP+, activation at pH 8.9 | Acetoanaerobium sticklandii |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.03 | - |
NADP+ | arsenate buffer, pH 7.4, two apparent Km values (0.03 mM and 0.07 mM) are determined for NADP+ because the Lineweaver-Burk plot is biphasic | Acetoanaerobium sticklandii | |
0.07 | - |
NADP+ | arsenate buffer, pH 7.4, two apparent Km values (0.03 mM and 0.07 mM) are determined for NADP+ because the Lineweaver-Burk plot is biphasic | Acetoanaerobium sticklandii | |
0.07 | - |
NADP+ | arsenate buffer, pH 9.1, two apparent Km values (0.07 mM and 0.27 mM) are determined for NADP+ because the Lineweaver-Burk plot is biphasic | Acetoanaerobium sticklandii | |
0.08 | - |
L-erythro-3,5-diaminohexanoate | pH 7.4, in presence of 0.4 mM NADP+ | Acetoanaerobium sticklandii | |
0.16 | - |
L-erythro-3,5-diaminohexanoate | pH 7.4, in presence of 0.4 mM NAD+ | Acetoanaerobium sticklandii | |
0.27 | - |
NADP+ | arsenate buffer, pH 9.1, two apparent Km values (0.07 mM and 0.27 mM) are determined for NADP+ because the Lineweaver-Burk plot is biphasic | Acetoanaerobium sticklandii | |
0.48 | - |
NAD+ | arsenate buffer, pH 7.4 | Acetoanaerobium sticklandii | |
0.67 | - |
NAD+ | arsenate buffer, pH 9.1 | Acetoanaerobium sticklandii | |
3.3 | - |
acetylpyridine-NAD+ | pH 8.8 | Acetoanaerobium sticklandii | |
22 | - |
deamino-NAD+ | pH 8.8 | Acetoanaerobium sticklandii |
Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
Ca2+ | 5 mM, 2.3fold activation at pH 8.9, inhibition at pH 7.8 | Acetoanaerobium sticklandii | |
Co2+ | 5 mM, 1.5fold activation at pH 8.9, inhibition at pH 7.8 | Acetoanaerobium sticklandii | |
Mn2+ | at pH 8.9 Mn2+ gives the highest increase in activity (232% at 0.5 mM). Concentrations of 0.01, 0.05, 0.1, 0.75, and 1.0 mM increase the enzyme activity by 110, 156, 188, 258, and 263%, respectively. The activity is maximal at 0.75 mM Mn2+ | Acetoanaerobium sticklandii | |
Zn2+ | 5 mM, 1.6fold activation at pH 8.9, inhibition at pH 7.8 | Acetoanaerobium sticklandii |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
39300 | - |
2 * 39300, SDS-PAGE | Acetoanaerobium sticklandii |
82000 | - |
gel filtration | Acetoanaerobium sticklandii |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
L-erythro-3,5-diaminohexanoate + H2O + NAD+ | Acetoanaerobium sticklandii | either NAD+ or NADP+ serve as coenzyme. Double-reciprocal plots of activity versus pyridine nucleotide concentration are biphasic. The Km values for NADP+ are lower but a higher Vmax is observed with NAD+ | 5-amino-3-oxohexanoate + NH3 + NADH + H+ | - |
? | |
L-erythro-3,5-diaminohexanoate + H2O + NADP+ | Acetoanaerobium sticklandii | either NAD+ or NADP+ serve as coenzyme. Double-reciprocal plots of activity versus pyridine nucleotide concentration are biphasic. The Km values for NADP+ are lower but a higher Vmax is observed with NAD+ | 5-amino-3-oxohexanoate + NH3 + NADPH + H+ | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Acetoanaerobium sticklandii | - |
- |
- |
Purification (Comment) | Organism |
---|---|
- |
Acetoanaerobium sticklandii |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
15.9 | - |
- |
Acetoanaerobium sticklandii |
Storage Stability | Organism |
---|---|
-20°C, 1 month, no loss of activity in crude enzyme extract | Acetoanaerobium sticklandii |
23°C, 1 day, no loss of activity in crude enzyme extract | Acetoanaerobium sticklandii |
4°C, 1 week, no loss of activity in crude enzyme extract | Acetoanaerobium sticklandii |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
L-erythro-3,5-diaminohexanoate + H2O + acetylpyridine-NAD+ | - |
Acetoanaerobium sticklandii | 5-amino-3-oxohexanoate + NH3 + acetylpyridine-NADH + H+ | - |
? | |
L-erythro-3,5-diaminohexanoate + H2O + deamino-NAD+ | - |
Acetoanaerobium sticklandii | 5-amino-3-oxohexanoate + NH3 + deamino-NADH + H+ | - |
? | |
L-erythro-3,5-diaminohexanoate + H2O + NAD+ | either NAD+ or NADP+ serve as coenzyme. Double-reciprocal plots of activity versus pyridine nucleotide concentration are biphasic. The Km values for NADP+ are lower but a higher Vmax is observed with NAD+ | Acetoanaerobium sticklandii | 5-amino-3-oxohexanoate + NH3 + NADH + H+ | - |
? | |
L-erythro-3,5-diaminohexanoate + H2O + NAD+ | Vmax/Km for NADP+ is 10-16fold higher, depending on KM-value | Acetoanaerobium sticklandii | 5-amino-3-oxohexanoate + NH3 + NADH + H+ | - |
? | |
L-erythro-3,5-diaminohexanoate + H2O + NADP+ | either NAD+ or NADP+ serve as coenzyme. Double-reciprocal plots of activity versus pyridine nucleotide concentration are biphasic. The Km values for NADP+ are lower but a higher Vmax is observed with NAD+ | Acetoanaerobium sticklandii | 5-amino-3-oxohexanoate + NH3 + NADPH + H+ | - |
? | |
L-erythro-3,5-diaminohexanoate + H2O + NADP+ | Vmax/Km for NADP+ is 10-16fold higher, depending on KM-value | Acetoanaerobium sticklandii | 5-amino-3-oxohexanoate + NH3 + NADPH + H+ | - |
? |
Subunits | Comment | Organism |
---|---|---|
dimer | 2 * 39300, SDS-PAGE | Acetoanaerobium sticklandii |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
24 | 40 | activity with NAD+, the activity of the enzyme increases up to 24°C, is relatively constant until 40°C and then decreases | Acetoanaerobium sticklandii |
52 | - |
activity with NADP+ increases up to 52°C and then rapidly falls off, probably due to heat inactivation of the protein | Acetoanaerobium sticklandii |
Temperature Minimum [°C] | Temperature Maximum [°C] | Comment | Organism |
---|---|---|---|
25 | 59 | 25°C: about 40% of maximal activity, 50°C: about 70% of maximal activity, cofactor: NAD+ | Acetoanaerobium sticklandii |
Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
---|---|---|---|
40 | - |
30 min, no loss of activity | Acetoanaerobium sticklandii |
50 | - |
t1/2: 56 min, inactivation follows first-order kinetics | Acetoanaerobium sticklandii |
55 | - |
t1/2: 32 min, inactivation follows first-order kinetics. In the presence of both NAD+ and 3,5-diaminohexanoate, the enzyme is effectively stabilized against heat inactivation at 55°C. NAD+ alone accelerates heat inactivation | Acetoanaerobium sticklandii |
59 | - |
t1/2: 4 min, inactivation follows first-order kinetics | Acetoanaerobium sticklandii |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
8.7 | 9 | activity with NAD+ or NADP+ | Acetoanaerobium sticklandii |
pH Minimum | pH Maximum | Comment | Organism |
---|---|---|---|
7.3 | 9.5 | pH 7.3: about 45% of maximal activity, pH 9.5: about 80% of maximal activity, cofactor: NAD+ | Acetoanaerobium sticklandii |
7.8 | 10.2 | pH 7.8: about 55% of maximal activity, pH 10.2: about 55% of maximal activity, cofactor: NADP+ | Acetoanaerobium sticklandii |
pH Stability | pH Stability Maximum | Comment | Organism |
---|---|---|---|
5 | 10.5 | 25°C, 30 min, no loss of activity | Acetoanaerobium sticklandii |
Cofactor | Comment | Organism | Structure |
---|---|---|---|
acetylpyridine-NAD+ | - |
Acetoanaerobium sticklandii | |
deamino-NAD+ | - |
Acetoanaerobium sticklandii | |
NAD+ | Vmax/Km for NADP+ is 10-16fold higher, depending on KM-value | Acetoanaerobium sticklandii | |
NADP+ | Vmax/Km for NADP+ is 10-16fold higher, depending on KM-value | Acetoanaerobium sticklandii |
Ki Value [mM] | Ki Value maximum [mM] | Inhibitor | Comment | Organism | Structure |
---|---|---|---|---|---|
0.0005 | - |
NADPH | pH 7.4 | Acetoanaerobium sticklandii | |
0.0195 | - |
NADH | pH 7.4 | Acetoanaerobium sticklandii | |
0.1 | - |
D-erythro-3,5-diaminohexanoate | pH 7.4 | Acetoanaerobium sticklandii | |
0.19 | - |
2,5-Diaminohexanoate | pH 7.4 | Acetoanaerobium sticklandii | |
0.52 | - |
2,4-Diaminopentanoate | pH 7.4 | Acetoanaerobium sticklandii | |
1 | - |
ATP | pH 7.4, with DL-erythro-3,5-diaminohexanoate as variable substrate | Acetoanaerobium sticklandii | |
2.5 | - |
L-lysine | pH 7.4 | Acetoanaerobium sticklandii | |
2.5 | - |
L-beta-Lysine | pH 7.4 | Acetoanaerobium sticklandii | |
4.5 | - |
ATP | pH 7.4, with NAD+ as variable substrate | Acetoanaerobium sticklandii | |
5.4 | - |
L-ornithine | pH 7.4 | Acetoanaerobium sticklandii | |
11.3 | - |
D-Lysine | pH 7.4 | Acetoanaerobium sticklandii | |
73.4 | - |
DL-beta-aminobutyrate | pH 7.4 | Acetoanaerobium sticklandii |