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Literature summary for 1.4.3.1 extracted from

  • Katane, M.; Furuchi, T.; Sekine, M.; Homma, H.
    Molecular cloning of a cDNA encoding mouse D-aspartate oxidase and functional characterization of its recombinant proteins by site-directed mutagenesis (2007), Amino Acids, 32, 69-78.
    View publication on PubMed
Search for more literature for 1.4.3.1

Cloned(Commentary)

Cloned (Comment) Organism
expressed in Escherichia coli strains BL21(DE3)pLysS and JM 109 Mus musculus

Protein Variants

Protein Variants Comment Organism
R216A mutant with significantly reduced activity Mus musculus
R216E mutant with significantly reduced activity Mus musculus
R216L mutant with significantly reduced activity Mus musculus
R216M mutant with significantly reduced activity Mus musculus
R237A mutant with significantly reduced activity Mus musculus
R237E mutant with significantly reduced activity Mus musculus
R237Y mutant with significantly reduced activity Mus musculus

Inhibitors

Inhibitors Comment Organism Structure
malate 52% inhibition at 50 mM Mus musculus
meso-tartrate 52% inhibition 50 mM Mus musculus
additional information benzoate has no inhibitory effect Mus musculus

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
37620
-
 calculated from amino acid sequence Mus musculus

Organism

Organism UniProt Comment Textmining
Mus musculus
-
-
-

Source Tissue

Source Tissue Comment Organism Textmining
kidney
-
 Mus musculus
-

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
D-asparagine + H2O + O2 15% activity compared to D-aspartate Mus musculus ?
-
 ?
D-aspartate + H2O + O2 100% activity Mus musculus oxaloacetate + NH3 + H2O2
-
 ?
D-glutamate + H2O + O2 14% activity compared to D-aspartate Mus musculus 2-oxoglutarate + NH3 + H2O2
-
 ?
D-methionine + H2O + O2 1.8% activity compared to D-aspartate Mus musculus 4-(methylsulfonyl)-2-oxobutanoic acid + NH3 + H2O2
-
 ?
additional information D-alanine,D-proline and L-asparagine are no substrates Mus musculus ?
-
 ?
N-methyl-D-aspartate + H2O + O2 83% activity compared to D-aspartate Mus musculus oxaloacetate + CH3NH2 + H2O2
-
 ?
N-methyl-L-aspartate + H2O + O2 4% activity compared to D-aspartate Mus musculus ?
-
 ?

Synonyms

Synonyms Comment Organism
D-Asp oxidase
-
 Mus musculus
DASPO
-
 Mus musculus

Cofactor

Cofactor Comment Organism Structure
FAD dependent Mus musculus
FMN 10% activity when FAD is replaced with FMN Mus musculus