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Literature summary for 1.4.3.11 extracted from

  • Sukhacheva, M.V.; Zhuravleva, N.I.
    Properties of and prospects for practical use of extracellular L-glutamate oxidase of Streptomyces sp. Z-11-6 (2004), Appl. Biochem. Microbiol., 40, 146-150.
No PubMed abstract available
Search for more literature for 1.4.3.11

Inhibitors

Inhibitors Comment Organism Structure
PCMB
-
 Streptomyces sp.

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.34
-
 L-glutamate
-
 Streptomyces sp.

Localization

Localization Comment Organism GeneOntology No. Textmining
extracellular
-
 Streptomyces sp.
-
-

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
22500
-
 alpha2beta2, 2 * 22500 + 2 * 25000, SDS-PAGE Streptomyces sp.
25000
-
 alpha2beta2, 2 * 22500 + 2 * 25000, SDS-PAGE Streptomyces sp.
95000
-
 gel filtration, non-denaturing PAGE Streptomyces sp.

Organism

Organism UniProt Comment Textmining
Streptomyces sp.
-
-
-
Streptomyces sp. Z-11-6
-
-
-

Purification (Commentary)

Purification (Comment) Organism
-
 Streptomyces sp.

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
L-glutamate + O2 + H2O
-
 Streptomyces sp. 2-oxoglutarate + NH3 + H2O2
-
 ?
L-glutamate + O2 + H2O
-
 Streptomyces sp. Z-11-6 2-oxoglutarate + NH3 + H2O2
-
 ?

Subunits

Subunits Comment Organism
tetramer alpha2beta2, 2 * 22500 + 2 * 25000, SDS-PAGE Streptomyces sp.

Temperature Stability [°C]

Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
50
-
 pH 4.5, 60 min, stable Streptomyces sp.
60
-
 pH 4.5, 15 min, stable Streptomyces sp.

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
5.5 8.5
-
 Streptomyces sp.

pH Stability

pH Stability pH Stability Maximum Comment Organism
4.5 9.5 60 min, 37°C, stable Streptomyces sp.

Cofactor

Cofactor Comment Organism Structure
FAD prosthetic group, enzyme:FAD ratio is 1:1 Streptomyces sp.