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Literature summary for 1.4.3.12 extracted from
- Characterization of a new cyclohexylamine oxidase from Acinetobacter sp. YT-02 (2018), Front. Microbiol., 9, 2848 .
Application
| Application | Comment | Organism |
|---|---|---|
| environmental protection | as a potential biocatalyst, the enzyme is promising in controlling cyclohexylamine pollution and deracemization of chiral amines | Acinetobacter sp. YT-02 |
Cloned(Commentary)
| Cloned (Comment) | Organism |
|---|---|
| expressed in Escherichia coli BL21 (DE3) | Acinetobacter sp. YT-02 |
Inhibitors
| Inhibitors | Comment | Organism | Structure |
|---|---|---|---|
| Ba2+ | 2 mM, 10% loss of activity | Acinetobacter sp. YT-02 |  |
| Co2+ | 2 mM, 15% loss of activity | Acinetobacter sp. YT-02 | |
| K+ | 2 mM, 17% loss of activity | Acinetobacter sp. YT-02 | |
| Mg2+ | 2 mM, 28% loss of activity | Acinetobacter sp. YT-02 | |
KM Value [mM]
| KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 0.25 | - |
Cyclohexylamine | pH 7.0, 30°C | Acinetobacter sp. YT-02 | |
| 0.25 | - |
Cyclohexylamine | pH 7.0, 50°C | Acinetobacter sp. YT-02 | |
Molecular Weight [Da]
| Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|
| 91000 | - |
gel filtration | Acinetobacter sp. YT-02 |
Natural Substrates/ Products (Substrates)
| Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| cyclohexylamine + O2 + H2O | Acinetobacter sp. YT-02 | - |
cyclohexanone + NH3 + H2O2 | - |
? | |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Acinetobacter sp. YT-02 | - |
- |
- |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
| Ni affinity chromatography | Acinetobacter sp. YT-02 |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| 2-methylcyclohexanamine + O2 + H2O | 22% of the activity with cyclohexylamine | Acinetobacter sp. YT-02 | 2-methylcyclohexanone + NH3 + H2O2 | - |
? | |
| 4-methylcyclohexanamine + O2 + H2O | 41% of the activity with cyclohexylamine | Acinetobacter sp. YT-02 | 4-methylcyclohexanone + NH3 + H2O2 | - |
? | |
| cycloheptylamine + O2 + H2O | 55% of the activity with cyclohexylamine | Acinetobacter sp. YT-02 | cycloheptanone + NH3 + H2O2 | - |
? | |
| cyclohexylamine + O2 + H2O | - |
Acinetobacter sp. YT-02 | cyclohexanone + NH3 + H2O2 | - |
? | |
| cyclopentylamine + O2 + H2O | 7% of the activity with cyclohexylamine | Acinetobacter sp. YT-02 | cyclopentanone + NH3 + H2O2 | - |
? | |
Subunits
| Subunits | Comment | Organism |
|---|---|---|
| dimer | - |
Acinetobacter sp. YT-02 |
Synonyms
| Synonyms | Comment | Organism |
|---|---|---|
| CF596_10820 | - |
Acinetobacter sp. YT-02 |
| CHAO | - |
Acinetobacter sp. YT-02 |
Temperature Optimum [°C]
| Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 50 | - |
- |
Acinetobacter sp. YT-02 |
Temperature Range [°C]
| Temperature Minimum [°C] | Temperature Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 30 | 60 | 30°C: about 70% of maximal activity, 60°C: about 70% of maximal activity | Acinetobacter sp. YT-02 |
Temperature Stability [°C]
| Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 40 | - |
30 min, pH 7.0, more than 40% activity of the enzyme is retained | Acinetobacter sp. YT-02 |
| 50 | - |
30 min, activity decreases rapidly when the temperature is higher than 50°C | Acinetobacter sp. YT-02 |
Turnover Number [1/s]
| Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 432 | - |
Cyclohexylamine | pH 7.0, 30°C | Acinetobacter sp. YT-02 | |
| 523 | - |
Cyclohexylamine | pH 7.0, 50°C | Acinetobacter sp. YT-02 | |
pH Optimum
| pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|
| 7 | - |
- |
Acinetobacter sp. YT-02 |
pH Range
| pH Minimum | pH Maximum | Comment | Organism |
|---|---|---|---|
| 7 | 9 | the enzyme retains more than 60% residual activity at pH 7.0-9.0 | Acinetobacter sp. YT-02 |
pH Stability
| pH Stability | pH Stability Maximum | Comment | Organism |
|---|---|---|---|
| 7 | 9 | enzyme activity is maintained at more than 50% level after incubation for 30 min at pH 7.0-9.0 | Acinetobacter sp. YT-02 |
kcat/KM [mM/s]
| kcat/KM Value [1/mMs-1] | kcat/KM Value Maximum [1/mMs-1] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 1724 | - |
Cyclohexylamine | pH 7.0, 30°C | Acinetobacter sp. YT-02 | |
| 2075 | - |
Cyclohexylamine | pH 7.0, 50°C | Acinetobacter sp. YT-02 | |
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