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Literature summary for 1.4.3.19 extracted from
- Purification and characterization of a novel glycine oxidase from Bacillus subtilis (1998), FEBS Lett., 438, 263-266.
Inhibitors
| Inhibitors | Comment | Organism | Structure |
|---|---|---|---|
| Ag2+ | 2.0 mM | Bacillus subtilis |  |
| Cd2+ | 2.0 mM | Bacillus subtilis | |
| Cu2+ | 2.0 mM | Bacillus subtilis | |
| Hg2+ | 2.0 mM | Bacillus subtilis | |
| PCMB | 2.0 mM | Bacillus subtilis | |
KM Value [mM]
| KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 0.22 | - |
sarcosine | - |
Bacillus subtilis | |
| 0.66 | - |
N-ethylglycine | - |
Bacillus subtilis | |
| 0.99 | - |
Gly | - |
Bacillus subtilis | |
| 46 | - |
D-Pro | - |
Bacillus subtilis | |
| 81 | - |
D-Ala | - |
Bacillus subtilis | |
Molecular Weight [Da]
| Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|
| 42000 | - |
4 * 42000, SDS-PAGE | Bacillus subtilis |
| 159000 | - |
gel filtration | Bacillus subtilis |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Bacillus subtilis | - |
- |
- |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
| recombinant enzyme expressed in Escherichia coli | Bacillus subtilis |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| D-2-aminobutyrate + H2O + O2 | 2.2% of the activity with sarcosine | Bacillus subtilis | ? + NH3 + H2O2 | - |
? | |
| D-Ala + H2O + O2 | 7.4% of the activity with sarcosine | Bacillus subtilis | pyruvate + NH3 + H2O2 | - |
? | |
| D-Pro + H2O + O2 | 15.1% of the activity with sarcosine | Bacillus subtilis | ? + NH3 + H2O2 | - |
? | |
| D-Val + H2O + O2 | 4.8% of the activity with sarcosine | Bacillus subtilis | ? + NH3 + H2O2 | - |
? | |
| glycine + H2O + O2 | 77.4% of the activity with sarcosine | Bacillus subtilis | glyoxylate + NH3 + H2O2 | - |
? | |
| N-ethylglycine + H2O + O2 | 85.3% of the activity with sarcosine | Bacillus subtilis | glyoxylate + ethylamine + H2O2 | - |
? | |
| N-methyl-D-Ala + H2O + O2 | 16.9% of the activity with sarcosine | Bacillus subtilis | ? + NH3 + H2O2 | - |
? | |
| sarcosine + H2O + O2 | - |
Bacillus subtilis | glyoxylate + methylamine + H2O2 | - |
? | |
Subunits
| Subunits | Comment | Organism |
|---|---|---|
| tetramer | 4 * 42000, SDS-PAGE | Bacillus subtilis |
Temperature Optimum [°C]
| Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 45 | - |
- |
Bacillus subtilis |
Temperature Stability [°C]
| Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 25 | - |
5 h, most stable between pH 7.5 and 8.5 | Bacillus subtilis |
| 45 | - |
10 min, stable up to | Bacillus subtilis |
Turnover Number [1/s]
| Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 1.1 | - |
D-Ala | - |
Bacillus subtilis | |
| 1.3 | - |
Gly | - |
Bacillus subtilis | |
| 1.3 | - |
D-Pro | - |
Bacillus subtilis | |
| 1.4 | - |
N-ethylglycine | - |
Bacillus subtilis | |
| 1.6 | - |
sarcosine | - |
Bacillus subtilis | |
pH Optimum
| pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|
| 8 | - |
- |
Bacillus subtilis |
pH Stability
| pH Stability | pH Stability Maximum | Comment | Organism |
|---|---|---|---|
| 7.5 | 8.5 | 25°C, 5 h, most stable | Bacillus subtilis |
Cofactor
| Cofactor | Comment | Organism | Structure |
|---|---|---|---|
| flavin | flavoprotein | Bacillus subtilis | |
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