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Literature summary for 1.4.3.19 extracted from

  • Martinez-Martinez, I.; Navarro-Fernandez, J.; Garcia-Carmona, F.; Takami, H.; Sanchez-Ferrer, A.
    Characterization and structural modeling of a novel thermostable glycine oxidase from Geobacillus kaustophilus HTA426 (2008), Proteins, 70, 1429-1441.
    View publication on PubMed

Cloned(Commentary)

Cloned (Comment) Organism
expressed in Escherichia coli Rosetta (DE3) cells Geobacillus kaustophilus

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.22
-
N-ethylglycine in 0.4 mM 4-aminoantipyrine, 7 mM phenol, 4.4 U/ml horseradish peroxidase, 0.0002 mM FAD, and 75 mM sodium diphosphate, at pH 8.5 and 37°C Geobacillus kaustophilus
0.25
-
glycine in 0.4 mM 4-aminoantipyrine, 7 mM phenol, 4.4 U/ml horseradish peroxidase, 0.0002 mM FAD, and 75 mM sodium diphosphate, at pH 8.5 and 37°C Geobacillus kaustophilus
0.45
-
sarcosine in 0.4 mM 4-aminoantipyrine, 7 mM phenol, 4.4 U/ml horseradish peroxidase, 0.0002 mM FAD, and 75 mM sodium diphosphate, at pH 8.5 and 37°C Geobacillus kaustophilus
0.52
-
glycine-ethyl-ester in 0.4 mM 4-aminoantipyrine, 7 mM phenol, 4.4 U/ml horseradish peroxidase, 0.0002 mM FAD, and 75 mM sodium diphosphate, at pH 8.5 and 37°C Geobacillus kaustophilus
9.3
-
D-proline in 0.4 mM 4-aminoantipyrine, 7 mM phenol, 4.4 U/ml horseradish peroxidase, 0.0002 mM FAD, and 75 mM sodium diphosphate, at pH 8.5 and 37°C Geobacillus kaustophilus
20.6
-
D-alanine in 0.4 mM 4-aminoantipyrine, 7 mM phenol, 4.4 U/ml horseradish peroxidase, 0.0002 mM FAD, and 75 mM sodium diphosphate, at pH 8.5 and 37°C Geobacillus kaustophilus
221.8
-
D-pipecolate in 0.4 mM 4-aminoantipyrine, 7 mM phenol, 4.4 U/ml horseradish peroxidase, 0.0002 mM FAD, and 75 mM sodium diphosphate, at pH 8.5 and 37°C Geobacillus kaustophilus

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
40000
-
4 * 40000, gel filtration Geobacillus kaustophilus
43000
-
4 * 43000, SDS-PAGE Geobacillus kaustophilus
160000
-
gel filtration Geobacillus kaustophilus
172000
-
SDS-PAGE Geobacillus kaustophilus

Organism

Organism UniProt Comment Textmining
Geobacillus kaustophilus
-
-
-

Purification (Commentary)

Purification (Comment) Organism
HiTrap FF chelating affinity column chromatography and phenyl-Sepharose FF hydrophobic column chromatography Geobacillus kaustophilus

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
1.75
-
crude extract, at 37°C and pH 8.5 Geobacillus kaustophilus
11.85
-
after 6.8fold purification, at 37°C and pH 8.5 Geobacillus kaustophilus

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
cephalosporin C + H2O + O2
-
Geobacillus kaustophilus ?
-
?
D-alanine + H2O + O2
-
Geobacillus kaustophilus pyruvate + NH3 + H2O2
-
?
D-arginine + H2O + O2
-
Geobacillus kaustophilus ?
-
?
D-aspartate + H2O + O2
-
Geobacillus kaustophilus ?
-
?
D-methionine + H2O + O2
-
Geobacillus kaustophilus 4-(methylsulfanyl)-2-oxobutanoic acid + NH3 + H2O2
-
?
D-norvaline + H2O + O2
-
Geobacillus kaustophilus 2-oxopentanoic acid + NH3 + H2O2
-
?
D-phenylalanine + H2O + O2
-
Geobacillus kaustophilus phenylpyruvate + NH3 + H2O2
-
?
D-pipecolate + H2O + O2
-
Geobacillus kaustophilus ?
-
?
D-proline + H2O + O2
-
Geobacillus kaustophilus ?
-
?
D-valine + H2O + O2
-
Geobacillus kaustophilus 3-methyl-2-oxobutanoic acid + NH3 + H2O2
-
?
glycine + H2O + O2
-
Geobacillus kaustophilus glyoxylate + NH3 + H2O2
-
?
glycine-ethyl-ester + H2O + O2
-
Geobacillus kaustophilus ?
-
?
additional information no activity with butilamine, benzilamine, D-histidine, D-leucine, L-proline, D-tryptophan, and D-tyrosine Geobacillus kaustophilus ?
-
?
N-ethylglycine + H2O + O2
-
Geobacillus kaustophilus glyoxylate + ethylamine + H2O2
-
?
sarcosine + H2O + O2
-
Geobacillus kaustophilus glyoxylate + methylamine + H2O2
-
?

Subunits

Subunits Comment Organism
homotetramer 4 * 43000, SDS-PAGE Geobacillus kaustophilus
homotetramer 4 * 40000, gel filtration Geobacillus kaustophilus

Synonyms

Synonyms Comment Organism
GOXK
-
Geobacillus kaustophilus

Temperature Stability [°C]

Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
60 70
-
Geobacillus kaustophilus
95
-
heat treatment of the protein at 95°C for 5 min results in the release of the FAD cofactor Geobacillus kaustophilus

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
8.5
-
-
Geobacillus kaustophilus

pH Stability

pH Stability pH Stability Maximum Comment Organism
4 9 a great decrease in stability is only produced at pH values below pH 4.0, shows good stability between pH 6.0 and 9.0 Geobacillus kaustophilus

Cofactor

Cofactor Comment Organism Structure
FAD flavoenzyme containing noncovalently bound FAD Geobacillus kaustophilus