Cloned (Comment) | Organism |
---|---|
expressed in Escherichia coli Rosetta (DE3) cells | Geobacillus kaustophilus |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.22 | - |
N-ethylglycine | in 0.4 mM 4-aminoantipyrine, 7 mM phenol, 4.4 U/ml horseradish peroxidase, 0.0002 mM FAD, and 75 mM sodium diphosphate, at pH 8.5 and 37°C | Geobacillus kaustophilus | |
0.25 | - |
glycine | in 0.4 mM 4-aminoantipyrine, 7 mM phenol, 4.4 U/ml horseradish peroxidase, 0.0002 mM FAD, and 75 mM sodium diphosphate, at pH 8.5 and 37°C | Geobacillus kaustophilus | |
0.45 | - |
sarcosine | in 0.4 mM 4-aminoantipyrine, 7 mM phenol, 4.4 U/ml horseradish peroxidase, 0.0002 mM FAD, and 75 mM sodium diphosphate, at pH 8.5 and 37°C | Geobacillus kaustophilus | |
0.52 | - |
glycine-ethyl-ester | in 0.4 mM 4-aminoantipyrine, 7 mM phenol, 4.4 U/ml horseradish peroxidase, 0.0002 mM FAD, and 75 mM sodium diphosphate, at pH 8.5 and 37°C | Geobacillus kaustophilus | |
9.3 | - |
D-proline | in 0.4 mM 4-aminoantipyrine, 7 mM phenol, 4.4 U/ml horseradish peroxidase, 0.0002 mM FAD, and 75 mM sodium diphosphate, at pH 8.5 and 37°C | Geobacillus kaustophilus | |
20.6 | - |
D-alanine | in 0.4 mM 4-aminoantipyrine, 7 mM phenol, 4.4 U/ml horseradish peroxidase, 0.0002 mM FAD, and 75 mM sodium diphosphate, at pH 8.5 and 37°C | Geobacillus kaustophilus | |
221.8 | - |
D-pipecolate | in 0.4 mM 4-aminoantipyrine, 7 mM phenol, 4.4 U/ml horseradish peroxidase, 0.0002 mM FAD, and 75 mM sodium diphosphate, at pH 8.5 and 37°C | Geobacillus kaustophilus |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
40000 | - |
4 * 40000, gel filtration | Geobacillus kaustophilus |
43000 | - |
4 * 43000, SDS-PAGE | Geobacillus kaustophilus |
160000 | - |
gel filtration | Geobacillus kaustophilus |
172000 | - |
SDS-PAGE | Geobacillus kaustophilus |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Geobacillus kaustophilus | - |
- |
- |
Purification (Comment) | Organism |
---|---|
HiTrap FF chelating affinity column chromatography and phenyl-Sepharose FF hydrophobic column chromatography | Geobacillus kaustophilus |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
1.75 | - |
crude extract, at 37°C and pH 8.5 | Geobacillus kaustophilus |
11.85 | - |
after 6.8fold purification, at 37°C and pH 8.5 | Geobacillus kaustophilus |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
cephalosporin C + H2O + O2 | - |
Geobacillus kaustophilus | ? | - |
? | |
D-alanine + H2O + O2 | - |
Geobacillus kaustophilus | pyruvate + NH3 + H2O2 | - |
? | |
D-arginine + H2O + O2 | - |
Geobacillus kaustophilus | ? | - |
? | |
D-aspartate + H2O + O2 | - |
Geobacillus kaustophilus | ? | - |
? | |
D-methionine + H2O + O2 | - |
Geobacillus kaustophilus | 4-(methylsulfanyl)-2-oxobutanoic acid + NH3 + H2O2 | - |
? | |
D-norvaline + H2O + O2 | - |
Geobacillus kaustophilus | 2-oxopentanoic acid + NH3 + H2O2 | - |
? | |
D-phenylalanine + H2O + O2 | - |
Geobacillus kaustophilus | phenylpyruvate + NH3 + H2O2 | - |
? | |
D-pipecolate + H2O + O2 | - |
Geobacillus kaustophilus | ? | - |
? | |
D-proline + H2O + O2 | - |
Geobacillus kaustophilus | ? | - |
? | |
D-valine + H2O + O2 | - |
Geobacillus kaustophilus | 3-methyl-2-oxobutanoic acid + NH3 + H2O2 | - |
? | |
glycine + H2O + O2 | - |
Geobacillus kaustophilus | glyoxylate + NH3 + H2O2 | - |
? | |
glycine-ethyl-ester + H2O + O2 | - |
Geobacillus kaustophilus | ? | - |
? | |
additional information | no activity with butilamine, benzilamine, D-histidine, D-leucine, L-proline, D-tryptophan, and D-tyrosine | Geobacillus kaustophilus | ? | - |
? | |
N-ethylglycine + H2O + O2 | - |
Geobacillus kaustophilus | glyoxylate + ethylamine + H2O2 | - |
? | |
sarcosine + H2O + O2 | - |
Geobacillus kaustophilus | glyoxylate + methylamine + H2O2 | - |
? |
Subunits | Comment | Organism |
---|---|---|
homotetramer | 4 * 43000, SDS-PAGE | Geobacillus kaustophilus |
homotetramer | 4 * 40000, gel filtration | Geobacillus kaustophilus |
Synonyms | Comment | Organism |
---|---|---|
GOXK | - |
Geobacillus kaustophilus |
Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
---|---|---|---|
60 | 70 | - |
Geobacillus kaustophilus |
95 | - |
heat treatment of the protein at 95°C for 5 min results in the release of the FAD cofactor | Geobacillus kaustophilus |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
8.5 | - |
- |
Geobacillus kaustophilus |
pH Stability | pH Stability Maximum | Comment | Organism |
---|---|---|---|
4 | 9 | a great decrease in stability is only produced at pH values below pH 4.0, shows good stability between pH 6.0 and 9.0 | Geobacillus kaustophilus |
Cofactor | Comment | Organism | Structure |
---|---|---|---|
FAD | flavoenzyme containing noncovalently bound FAD | Geobacillus kaustophilus |