General Stability | Organism |
---|---|
freezing, quick freezing with dry ice/acetone mixture and storage at -15°C for 60 h, enzyme is stable | Ophiophagus hannah |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
additional information | substrate inhibition | Ophiophagus hannah |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.14 | - |
L-leucine | - |
Ophiophagus hannah |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
68000 | - |
2 * 68000, SDS-PAGE | Ophiophagus hannah |
140000 | - |
gel filtration | Ophiophagus hannah |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Ophiophagus hannah | - |
king cobra | - |
Posttranslational Modification | Comment | Organism |
---|---|---|
glycoprotein | 3.8% carbohydrate | Ophiophagus hannah |
Purification (Comment) | Organism |
---|---|
- |
Ophiophagus hannah |
Source Tissue | Comment | Organism | Textmining |
---|---|---|---|
venom | - |
Ophiophagus hannah | - |
Storage Stability | Organism |
---|---|
4°C, pH 7.4, 20 mM Tris-HCl, 3 months | Ophiophagus hannah |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
L-amino acid + H2O + O2 | - |
Ophiophagus hannah | 2-oxo acid + NH3 + H2O2 | - |
? |
Subunits | Comment | Organism |
---|---|---|
dimer | 2 * 68000, SDS-PAGE | Ophiophagus hannah |
Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
---|---|---|---|
4 | - |
3 months, no loss of activity | Ophiophagus hannah |
25 | - |
1 month, no loss of activity | Ophiophagus hannah |
37 | - |
5 days, no loss of activity, 14 days, 20% loss of activity | Ophiophagus hannah |
Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
86.6 | - |
L-leucine | - |
Ophiophagus hannah |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
8.5 | - |
- |
Ophiophagus hannah |
pH Stability | pH Stability Maximum | Comment | Organism |
---|---|---|---|
9 | - |
25°C, 1 h stable | Ophiophagus hannah |
9 | - |
25°C, 5 min, 47% loss of activity, complete loss of activity after 30 min | Ophiophagus hannah |
Cofactor | Comment | Organism | Structure |
---|---|---|---|
FAD | 2 mol of FAD per mol of enzyme | Ophiophagus hannah |