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Literature summary for 1.4.3.2 extracted from

  • Coudert, M.; Vandecasteele, J.P.
    Charcterization and physiological function of a soluble L-amino acid oxidase in Corynebacterium (1975), Arch. Microbiol., 102, 151-153.
    View publication on PubMed

General Stability

General Stability Organism
2-mercaptoethanol stabilizes Corynebacterium sp.
KCl stabilizes Corynebacterium sp.

Inhibitors

Inhibitors Comment Organism Structure
additional information substrate inhibition Corynebacterium sp.

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.011
-
L-phenylalanine
-
Corynebacterium sp.
0.04
-
L-lysine
-
Corynebacterium sp.
0.05
-
cysteine
-
Corynebacterium sp.

Localization

Localization Comment Organism GeneOntology No. Textmining
soluble
-
Corynebacterium sp.
-
-

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
130000 140000 gel filtration Corynebacterium sp.

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
L-amino acid + H2O + O2 Corynebacterium sp. utilization of amino acids as nitrogen source 2-oxo acid + NH3 + H2O2
-
?
L-amino acid + H2O + O2 Corynebacterium sp. A20 utilization of amino acids as nitrogen source 2-oxo acid + NH3 + H2O2
-
?

Organism

Organism UniProt Comment Textmining
Corynebacterium sp.
-
A20
-
Corynebacterium sp. A20
-
A20
-
no activity in Bacillus subtilis subsp. subtilis
-
-
-
no activity in Bacillus subtilis subsp. subtilis NCIB 3610
-
-
-
no activity in Escherichia coli
-
-
-
no activity in Pseudomonas putida
-
-
-
no activity in Pseudomonas putida T1
-
-
-

Storage Stability

Storage Stability Organism
4°C, 66 mM KCl, 10 mM 2-mercaptoethanol, 1 month, 5-10% loss of activity Corynebacterium sp.

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
L-alanine + H2O + O2
-
Corynebacterium sp. 2-oxopropanoic acid + NH3 + H2O2
-
?
L-alpha-aminobutyric acid + H2O + O2
-
Corynebacterium sp. alpha-ketobutyrate + NH3 + H2O2
-
?
L-amino acid + H2O + O2
-
Corynebacterium sp. 2-oxo acid + NH3 + H2O2
-
?
L-amino acid + H2O + O2 utilization of amino acids as nitrogen source Corynebacterium sp. 2-oxo acid + NH3 + H2O2
-
?
L-amino acid + H2O + O2
-
Corynebacterium sp. A20 2-oxo acid + NH3 + H2O2
-
?
L-amino acid + H2O + O2 utilization of amino acids as nitrogen source Corynebacterium sp. A20 2-oxo acid + NH3 + H2O2
-
?
L-arginine + H2O + O2
-
Corynebacterium sp. 2-oxo-5-guanidinovaleric acid + NH3 + H2O2
-
?
L-cysteine + H2O + O2
-
Corynebacterium sp. 2-oxo-3-mercaptopropanoic acid + NH3 + H2O2
-
?
L-histidine + H2O + O2
-
Corynebacterium sp. 3-(1H-imidazol-4-yl)-2-oxopropionic acid + NH3 + H2O2
-
?
L-isoleucine + H2O + O2
-
Corynebacterium sp. alpha-keto-beta-methyl-pentanoic acid + NH3 + H2O2
-
?
L-leucine + H2O + O2
-
Corynebacterium sp. 4-methyl-2-oxopentanoic acid + NH3 + H2O2
-
?
L-lysine + H2O + O2
-
Corynebacterium sp. 6-amino-2-oxohexanoic acid + NH3 + H2O2
-
?
L-methionine + H2O + O2
-
Corynebacterium sp. 4-methylsulfanyl-2-oxobutanoate + NH3 + H2O2
-
?
L-ornithine + H2O + O2
-
Corynebacterium sp. 2-oxo-5-aminopentanoate + NH3 + H2O2
-
?
L-phenylalanine + H2O + O2
-
Corynebacterium sp. phenylpyruvate + NH3 + H2O2
-
?
L-phenylalanine + H2O + O2
-
Corynebacterium sp. A20 phenylpyruvate + NH3 + H2O2
-
?
L-serine + H2O + O2
-
Corynebacterium sp. 2-oxo-3-hydroxypropionic acid + NH3 + H2O2
-
?
L-tryptophan + H2O + O2
-
Corynebacterium sp. indole-3-pyruvate + NH3 + H2O2
-
?
L-tyrosine + H2O + O2
-
Corynebacterium sp. p-hydroxyphenylpyruvate + NH3 + H2O2
-
?
additional information not: proline Corynebacterium sp. ?
-
?
additional information not: L-threonine Corynebacterium sp. ?
-
?
additional information not: proline Corynebacterium sp. A20 ?
-
?
additional information not: L-threonine Corynebacterium sp. A20 ?
-
?