Localization | Comment | Organism | GeneOntology No. | Textmining |
---|---|---|---|---|
extracellular | venom | Bothrops atrox | - |
- |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
67000 | - |
x * 67000, SDS-PAGE | Bothrops atrox |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Bothrops atrox | - |
- |
- |
Posttranslational Modification | Comment | Organism |
---|---|---|
glycoprotein | - |
Bothrops atrox |
Purification (Comment) | Organism |
---|---|
native enzyme 54fold from venom by gel filtration and ultrafiltration, anion exchange chromatography, and lectin affinity chromatography to homogeneity | Bothrops atrox |
Source Tissue | Comment | Organism | Textmining |
---|---|---|---|
venom | - |
Bothrops atrox | - |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
87.5 | - |
purified enzyme, substrate L-Leu | Bothrops atrox |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
L-Ile + H2O + O2 | - |
Bothrops atrox | 3-methyl-2-oxopentanoate + NH3 + H2O2 | - |
? | |
L-Leu + H2O + O2 | - |
Bothrops atrox | 4-methyl-2-oxopentanoic acid + NH3 + H2O2 | - |
? | |
L-Met + H2O + O2 | best substrate | Bothrops atrox | 4-methylsulfanyl-2-oxobutanoate + NH3 + H2O2 | - |
? | |
L-Phe + H2O + O2 | - |
Bothrops atrox | phenylpyruvate + NH3 + H2O2 | - |
? | |
L-Trp + H2O + O2 | - |
Bothrops atrox | indol-3-pyruvate + NH3 + H2O2 | - |
? | |
L-Tyr + H2O + O2 | - |
Bothrops atrox | 4-hydroxyphenylpyruvate + NH3 + H2O2 | - |
? | |
additional information | substrate specificity, overview | Bothrops atrox | ? | - |
? |
Subunits | Comment | Organism |
---|---|---|
? | x * 67000, SDS-PAGE | Bothrops atrox |
More | peptide mapping | Bothrops atrox |
Synonyms | Comment | Organism |
---|---|---|
LAAO | - |
Bothrops atrox |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
7.2 | - |
assay at | Bothrops atrox |
Organism | Comment | pI Value Maximum | pI Value |
---|---|---|---|
Bothrops atrox | isoelectric focussing | - |
4.4 |
General Information | Comment | Organism |
---|---|---|
physiological function | the enzyme induces in vitro platelet aggregation, which may be due to H2O2 production, the addition of catalase completely inhibits the aggregation effect. It also shows cytotoxicity towards several cancer cell lines: HL60, Jurkat, B16F10 and PC12 causing apoptosis. The cytotoxicity activity is abolished by catalase | Bothrops atrox |