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Literature summary for 1.4.3.2 extracted from
- Discovery of serum L-amino acid oxidase in the rockfish Sebastes schlegeli: isolation and biochemical characterization (2010), Comp. Biochem. Physiol. B, 157, 351-356.
KM Value [mM]
| KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 0.37 | - |
L-lysine | pH 4.0, 37°C | Sebastes schlegelii |  |
Molecular Weight [Da]
| Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|
| 53000 | - |
x * 53000, SDS-APGE | Sebastes schlegelii |
| 160000 | - |
gel filtration | Sebastes schlegelii |
Natural Substrates/ Products (Substrates)
| Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| L-lysine + H2O + O2 | Sebastes schlegelii | strictly specific for | 6-amino-2-oxohexanoic acid + NH3 + H2O2 | - |
? | |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Sebastes schlegelii | - |
- |
- |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
| native enzyme 700fold from serum by concanavalin A lectin affinity chromatography, anion exchange HPLC, hydroxyapatite HPLC and gel filtration HPLC | Sebastes schlegelii |
Source Tissue
| Source Tissue | Comment | Organism | Textmining |
|---|---|---|---|
| serum | - |
Sebastes schlegelii | - |
Specific Activity [micromol/min/mg]
| Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
|---|---|---|---|
| 26 | - |
purified native enzyme, pH 4.0, 37°C | Sebastes schlegelii |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| L-lysine + H2O + O2 | strictly specific for | Sebastes schlegelii | 6-amino-2-oxohexanoic acid + NH3 + H2O2 | - |
? | |
Subunits
| Subunits | Comment | Organism |
|---|---|---|
| dimer or trimer | x * 53000, SDS-APGE | Sebastes schlegelii |
Synonyms
| Synonyms | Comment | Organism |
|---|---|---|
| LAO | - |
Sebastes schlegelii |
Temperature Optimum [°C]
| Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 37 | - |
assay at | Sebastes schlegelii |
Turnover Number [1/s]
| Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 57.1 | - |
L-lysine | pH 4.0, 37°C | Sebastes schlegelii | |
pH Optimum
| pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|
| 4 | - |
assay at | Sebastes schlegelii |
Cofactor
| Cofactor | Comment | Organism | Structure |
|---|---|---|---|
| FAD | - |
Sebastes schlegelii | |
General Information
| General Information | Comment | Organism |
|---|---|---|
| physiological function | serum LAO is involved in innate immunity in the rockfish body, it exhibits a broad antibacterial activity against Gram positive and negative bacteria, most potently against Aeromonas hydrophila and Aeromonas salmonicida with a minimum inhibitory concentration of 0.000078 mg/ml | Sebastes schlegelii |
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Release 2023.1 (January 2023)
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