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Literature summary for 1.4.3.2 extracted from

  • Isobe, K.; Tamauchi, H.; Fuhshuku, K.; Nagasawa, S.; Asano, Y.
    A simple enzymatic method for production of a wide variety of D-amino acids using L-amino acid oxidase from Rhodococcus sp. AIU Z-35-1 (2010), Enzyme Res., 2010, 567210.
    View publication on PubMedView publication on EuropePMC
Search for more literature for 1.4.3.2

Protein Variants

Protein Variants Comment Organism
additional information development of a simple enzymatic method for production of a wide variety of D-amino acids using L-amino acid oxidase from Rhodococcus sp. AIU Z-35-1, overview Rhodococcus sp.

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
L-lysine + H2O + O2 Rhodococcus sp.
-
 6-amino-2-oxohexanoic acid + NH3 + H2O2
-
 ?
L-lysine + H2O + O2 Rhodococcus sp. AIU Z-35-1
-
 6-amino-2-oxohexanoic acid + NH3 + H2O2
-
 ?

Organism

Organism UniProt Comment Textmining
Rhodococcus sp.
-
-
-
Rhodococcus sp. AIU Z-35-1
-
-
-

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
L-arginine + H2O + O2
-
 Rhodococcus sp. 2-oxo-5-guanidinopentanoic acid + NH3 + H2O2
-
 ?
L-arginine + H2O + O2
-
 Rhodococcus sp. AIU Z-35-1 2-oxo-5-guanidinopentanoic acid + NH3 + H2O2
-
 ?
L-citrulline + H2O + O2
-
 Rhodococcus sp. 2-oxo-5-ureidovaleric acid + NH3 + H2O2
-
 ?
L-glutamate + H2O + O2
-
 Rhodococcus sp. 2-oxoglutaric acid + NH3 + H2O2
-
 ?
L-glutamine + H2O + O2
-
 Rhodococcus sp. 2-oxo-4-carbamoylbutanoic acid + NH3 + H2O2
-
 ?
L-glutamine + H2O + O2
-
 Rhodococcus sp. AIU Z-35-1 2-oxo-4-carbamoylbutanoic acid + NH3 + H2O2
-
 ?
L-histidine + H2O + O2
-
 Rhodococcus sp. 2-oxo-4-imidazolepropionic acid + NH3 + H2O2
-
 ?
L-homoserine + H2O + O2
-
 Rhodococcus sp. 2-oxo-4-hydroxybutyric acid + NH3 + H2O2
-
 ?
L-leucine + H2O + O2
-
 Rhodococcus sp. 2-oxo-4-methylvaleric acid + NH3 + H2O2
-
 ?
L-lysine + H2O + O2
-
 Rhodococcus sp. 6-amino-2-oxohexanoic acid + NH3 + H2O2
-
 ?
L-lysine + H2O + O2
-
 Rhodococcus sp. AIU Z-35-1 6-amino-2-oxohexanoic acid + NH3 + H2O2
-
 ?
L-phenylalanine + H2O + O2
-
 Rhodococcus sp. phenylpyruvate + NH3 + H2O2
-
 ?
L-phenylalanine + H2O + O2
-
 Rhodococcus sp. AIU Z-35-1 phenylpyruvate + NH3 + H2O2
-
 ?
additional information the enzyme from Rhodococcus sp. AIU Z-35-1 shows broad substrate specificity Rhodococcus sp. ?
-
 ?
additional information the enzyme from Rhodococcus sp. AIU Z-35-1 shows broad substrate specificity Rhodococcus sp. AIU Z-35-1 ?
-
 ?
Nepsilon-acetyl-L-lysine + H2O + O2
-
 Rhodococcus sp. 2-oxo-6-acetylaminohexanoic acid + NH3 + H2O2
-
 ?

Synonyms

Synonyms Comment Organism
L-AAO
-
 Rhodococcus sp.

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
30
-
 assay at Rhodococcus sp.

Temperature Stability [°C]

Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
30 50 pH 7.0, 60 min, stable up to 30°C, 80% of the enzyme activity remains at 40°C, the enzyme activity is almost completely lost at 50°C Rhodococcus sp.

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
7
-
 assay at Rhodococcus sp.

pH Range

pH Minimum pH Maximum Comment Organism
5.5 8.5 activity assay range Rhodococcus sp.

pH Stability

pH Stability pH Stability Maximum Comment Organism
5.5 7 40°C, 60 min, loss of about 20-30% activity Rhodococcus sp.
7 8.5 40°C, 60 min, highly stable Rhodococcus sp.