Cloned (Comment) | Organism |
---|---|
overexpression of His-tagged wild-type and mutants enzymes in Escherichia coli strain Rosetta2(DE3) | Aspergillus niger |
Crystallization (Comment) | Organism |
---|---|
purified recombinant genetic variants MAO-N-3 and MAO-N-5, from 10% w/v PEG 3350, 0.2 M proline, 0.1 M HEPES, pH 7.5, or 10% w/v PEG 5000 MME, 5% v/v tacsimate, 0.1 M HEPES, pH 7.0, with no difference in diffraction quality between the crystals from the two conditions, X-ray diffraction structure determination and analysis at 2.45 A and 1.85 A resolution, respectively | Aspergillus niger |
Protein Variants | Comment | Organism |
---|---|---|
additional information | genetic variants of MAO-N produced by directed evolution possess altered substrate specificity, e.g. MAO-N-3 and MAO-N-5 | Aspergillus niger |
N336S/M348K/I246M | genetic variant MOA-N-3 exhibits improved activity towards a range of amine substrates compared to the wild-type enzyme, including chiral secondary amines | Aspergillus niger |
N336S/M348K/I246M/T384N/D385S | genetic variant MOA-N-5 shows improved activity and enantioselectivity towards a broad range of tertiary amines compared to the wild-type enzyme | Aspergillus niger |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
rasagiline ethanedisulfonate | inhibits MAO-B | Aspergillus niger | |
tacsimate | - |
Aspergillus niger |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Aspergillus niger | - |
- |
- |
Purification (Comment) | Organism |
---|---|
recombinant His-tagged wild-type and mutants enzymes from Escherichia coli strain Rosetta2(DE3), removal of the His tag | Aspergillus niger |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
additional information | MAO-N is an FAD-dependent enzyme that catalyses the conversion of terminal amines to their corresponding aldehyde | Aspergillus niger | ? | - |
? |
Subunits | Comment | Organism |
---|---|---|
tetramer | - |
Aspergillus niger |
Synonyms | Comment | Organism |
---|---|---|
MAO-N | - |
Aspergillus niger |
Cofactor | Comment | Organism | Structure |
---|---|---|---|
FAD | dependent on | Aspergillus niger |