Cloned (Comment) | Organism |
---|---|
expressed in Saccharomyces cerevisiae INVSC-1 cells | Ogataea angusta |
Crystallization (Comment) | Organism |
---|---|
isoform HPAO-2, sitting drop vapor diffusion method, using 0.5-0.75 M potassium sodium tartrate tetrahydrate in 0.10 M phosphate, pH 6.0-7.5, at 20°C | Ogataea angusta |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
72000 | - |
x * 72000, SDS-PAGE | Ogataea angusta |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Ogataea angusta | P12807 | - |
- |
Purification (Comment) | Organism |
---|---|
Q sepharose column chromatography | Ogataea angusta |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
benzylamine + H2O + O2 | - |
Ogataea angusta | benzaldehyde + NH3 + H2O2 | - |
? | |
methylamine + H2O + O2 | - |
Ogataea angusta | formaldehyde + NH3 + H2O2 | - |
? | |
additional information | isoform HPAO-2 shows a clear preference for bulkier aromatic amines and isoform HPAO-1 shows a preference for short aliphatic amines | Ogataea angusta | ? | - |
? |
Subunits | Comment | Organism |
---|---|---|
? | x * 72000, SDS-PAGE | Ogataea angusta |
Synonyms | Comment | Organism |
---|---|---|
CAO | - |
Ogataea angusta |
Copper amine oxidase | - |
Ogataea angusta |
hPAO-1 | isoform | Ogataea angusta |
HPAO-2 | isoform | Ogataea angusta |
Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.066 | - |
benzylamine | isoform HPAO-1, at 25°C, in 100 mM potassium phosphate, pH 7.2 | Ogataea angusta | |
2.18 | - |
methylamine | isoform HPAO-2, at 25°C, in 100 mM potassium phosphate, pH 7.2 | Ogataea angusta | |
6.2 | - |
methylamine | isoform HPAO-1, at 25°C, in 100 mM potassium phosphate, pH 7.2 | Ogataea angusta | |
8.1 | - |
benzylamine | isoform HPAO-2, at 25°C, in 100 mM potassium phosphate, pH 7.2 | Ogataea angusta |
Cofactor | Comment | Organism | Structure |
---|---|---|---|
2,4,5-trihydroxyphenylalanine quinone | - |
Ogataea angusta |
kcat/KM Value [1/mMs-1] | kcat/KM Value Maximum [1/mMs-1] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.09 | - |
benzylamine | isoform HPAO-1, at 25°C, in 100 mM potassium phosphate, pH 7.2 | Ogataea angusta | |
1.2 | - |
methylamine | isoform HPAO-2, at 25°C, in 100 mM potassium phosphate, pH 7.2 | Ogataea angusta | |
30 | - |
methylamine | isoform HPAO-1, at 25°C, in 100 mM potassium phosphate, pH 7.2 | Ogataea angusta | |
81 | - |
O2 | isoform HPAO-1, using benzylamine as cosubstrate, at 25°C, in 100 mM potassium phosphate, pH 7.2 | Ogataea angusta | |
160 | - |
O2 | isoform HPAO-2, using methylamine as cosubstrate, at 25°C, in 100 mM potassium phosphate, pH 7.2 | Ogataea angusta | |
400 | - |
O2 | isoform HPAO-1, using methylamine as cosubstrate, at 25°C, in 100 mM potassium phosphate, pH 7.2 | Ogataea angusta | |
470 | - |
O2 | isoform HPAO-2, using benzylamine as cosubstrate, at 25°C, in 100 mM potassium phosphate, pH 7.2 | Ogataea angusta | |
900 | - |
benzylamine | isoform HPAO-2, at 25°C, in 100 mM potassium phosphate, pH 7.2 | Ogataea angusta |