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Literature summary for 1.4.3.22 extracted from

  • Heim, W.G.; Sykes, K.A.; Hildreth, S.B.; Sun, J.; Lu, R.H.; Jelesko, J.G.
    Cloning and characterization of a Nicotiana tabacum methylputrescine oxidase transcript (2006), Phytochemistry, 68, 454-463.
    View publication on PubMed
Search for more literature for 1.4.3.22

Cloned(Commentary)

Cloned (Comment) Organism
expressed in Escherichia coli Rosetta cells as a recombinant thioredoxin-His6-MPO1 fusion protein, using the plasmid pJGJ389. The recombinant MPO1 protein utilizes N-methylputrescine more efficiently than other diamines, a homology-based cloning strategy Nicotiana tabacum

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
additional information
-
 additional information the kinetic properties of the MPO1 enzyme may play an important role in determining the pyridine alkaloid profiles observed in tobacco roots Nicotiana tabacum
0.19
-
 N-methylputrescine Vmax: 28.8 nkat, pH and temperature not specified in the publication Nicotiana tabacum
0.35
-
 1,3-diaminopropane Vmax: 11.3 nkat, pH and temperature not specified in the publication Nicotiana tabacum
0.76
-
 1,4-diaminobutane Vmax: 11.1 nkat, pH and temperature not specified in the publication Nicotiana tabacum
1.79
-
 cadaverine Vmax: 5.2 nkat, pH and temperature not specified in the publication Nicotiana tabacum

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
106000
-
 thioredoxin-His6-MPO1 fusion protein Nicotiana tabacum

Organism

Organism UniProt Comment Textmining
Nicotiana tabacum
-
-
-

Source Tissue

Source Tissue Comment Organism Textmining
hairy root culture
-
 Nicotiana tabacum
-

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1,3-diaminopropane + H2O + O2
-
 Nicotiana tabacum 3-aminopropanal + NH3 + H2O2
-
 ?
1,4-diaminobutane + H2O + O2
-
 Nicotiana tabacum 4-aminobutanal + NH3 + H2O2
-
 ?
cadaverine + H2O + O2
-
 Nicotiana tabacum 5-aminopentanal + NH3 + H2O2
-
 ?
N-methylputrescine + H2O + O2
-
 Nicotiana tabacum 4-(methylamino)butanal + NH3 + H2O2
-
 ?

Synonyms

Synonyms Comment Organism
methylputrescine oxidase
-
 Nicotiana tabacum
MPO
-
 Nicotiana tabacum
pWGH15
-
 Nicotiana tabacum

General Information

General Information Comment Organism
physiological function the oxidative deamination is an essential step in both pyridine and tropane alkaloid biosynthesis. MPO is part of a multigene family Nicotiana tabacum