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Literature summary for 1.4.3.22 extracted from
- Purification of diamine oxidase and its properties in germinated fava bean (Vicia faba L.) (2012), J. Sci. Food Agric., 92, 1709-1715.
Inhibitors
| Inhibitors | Comment | Organism | Structure |
|---|---|---|---|
| 2-mercaptoethanol | 2.94% residual activity at 0.5 mM | Vicia faba |  |
| aminoguanidine | 3.92% residual activity at 0.25 mM | Vicia faba | |
| Cu2+ | 7.84% residual activity at 3 mM | Vicia faba | |
| EDTA-Na2 | 76.47% residual activity at 1 mM | Vicia faba | |
| EGTA | 71.57% residual activity at 1 mM | Vicia faba | |
| Fe3+ | 10.78% residual activity at 3 mM | Vicia faba | |
| L-cysteine | complete inhibition at 0.1 mM | Vicia faba | |
| Mg2+ | 78.43% residual activity at 3 mM | Vicia faba | |
KM Value [mM]
| KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 0.23 | - |
putrescine | at pH 6.5 and 40°C | Vicia faba | |
| 0.96 | - |
spermidine | at pH 6.5 and 40°C | Vicia faba | |
Metals/Ions
| Metals/Ions | Comment | Organism | Structure |
|---|---|---|---|
| additional information | not influenced by Ca2+, Zn2+, Mn2+, and Ba2+ | Vicia faba |
Molecular Weight [Da]
| Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|
| 52000 | - |
2 * 52000, SDS-PAGE | Vicia faba |
| 121000 | - |
gel filtration | Vicia faba |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Vicia faba | - |
cultivar Qidou 2 | - |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
| ammonium sulfate precipitation, DEAE-Sepharose column chromatography, and Sephadex G-100 gel filtration | Vicia faba |
Source Tissue
| Source Tissue | Comment | Organism | Textmining |
|---|---|---|---|
| seed | - |
Vicia faba | - |
| shoot | - |
Vicia faba | - |
Specific Activity [micromol/min/mg]
| Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
|---|---|---|---|
| additional information | - |
the crude extract has a specific activity of 9.1 units/mg, while the 215fold purified enzyme has a specific activity of 1962.47 units/mg. A change in absorbance at 555 nm of 0.01 per minute is considered as one unit of enzyme activity | Vicia faba |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| additional information | the enzyme does not degrade spermine | Vicia faba | ? | - |
? | |
| putrescine + H2O + O2 | - |
Vicia faba | ? | - |
? | |
| spermidine + H2O + O2 | - |
Vicia faba | ? | - |
? | |
Subunits
| Subunits | Comment | Organism |
|---|---|---|
| homodimer | 2 * 52000, SDS-PAGE | Vicia faba |
Synonyms
| Synonyms | Comment | Organism |
|---|---|---|
| DAO | - |
Vicia faba |
| EC 1.4.3.6 | formerly | Vicia faba |
Temperature Optimum [°C]
| Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 40 | - |
- |
Vicia faba |
Temperature Stability [°C]
| Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 30 | 60 | the enzyme retains its activity after being incubated at 30°C for 30 min, the enzyme is rapidly deactivated above 45°C and reaches almost zero activity at 60°C | Vicia faba |
pH Optimum
| pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|
| 6.5 | - |
- |
Vicia faba |
Expression
| Organism | Comment | Expression |
|---|---|---|
| Vicia faba | enzyme activity in the shoot is undetectable up to 2 days of germination but then increases significantly | up |
General Information
| General Information | Comment | Organism |
|---|---|---|
| metabolism | diamine oxidase is one of the key enzymes for gamma-aminobutyric acid formation | Vicia faba |
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