Literature summary for 1.4.3.3 extracted from

Pollegioni, L.; Fukui, K.; Massey, V.
Studies on the kinetic mechanism of pig kidney D-amino acid oxidase by site-directed mutagenesis of tyrosine 224 and tyrosine 228 (1994), J. Biol. Chem., 269, 31666-31673.

Search for more literature for 1.4.3.3

Cloned(Commentary)

Cloned (Comment)	Organism
-	Sus scrofa

Protein Variants

Protein Variants	Comment	Organism
Y224F	turnover numbers similar to wild-type	Sus scrofa
Y228F	turnover numbers lower than turnover numbers of wild-type	Sus scrofa

KM Value [mM]

KM Value [mM]	KM Value Maximum [mM]	Substrate	Comment	Organism	Structure
2.3	-	D-alanine	wild-type	Sus scrofa
2.8	-	D-alanine	mutant Y224F	Sus scrofa
7.5	-	D-phenylalanine	mutant Y224F	Sus scrofa
9.1	-	D-proline	mutant Y224F	Sus scrofa
9.5	-	D-proline	wild-type	Sus scrofa
12.2	-	D-proline	mutant Y228F	Sus scrofa
15.6	-	D-methionine	mutant Y224F	Sus scrofa
18.3	-	D-phenylalanine	mutant Y228F	Sus scrofa
27	-	D-alanine	mutant Y228F	Sus scrofa
50	-	D-methionine	mutant Y228F	Sus scrofa
55.9	-	D-phenylalanine	wild-type	Sus scrofa
143	-	D-methionine	wild-type	Sus scrofa

Natural Substrates/ Products (Substrates)

Natural Substrates	Organism	Comment (Nat. Sub.)	Natural Products	Comment (Nat. Pro.)	Rev.	Reac.
D-alanine + H2O + O2	Sus scrofa	-	pyruvic acid + NH3 + H2O2	-	?
D-methionine + H2O + O2	Sus scrofa	-	4-methylthio-2-oxobutanoic acid + NH3 + H2O2	-	?
D-phenylalanine + H2O + O2	Sus scrofa	-	phenylpyruvic acid + NH3 + H2O2	-	?
D-proline + H2O + O2	Sus scrofa	-	2-oxopentanoic acid + NH3 + H2O2	-	?

Organism

Organism	UniProt	Comment	Textmining
Sus scrofa	-	-	-

Purification (Commentary)

Purification (Comment)	Organism
-	Sus scrofa

Source Tissue

Source Tissue	Comment	Organism	Textmining
kidney	-	Sus scrofa	-

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg]	Specific Activity Maximum [µmol/min/mg]	Comment	Organism
0.15	-	-	Sus scrofa

Substrates and Products (Substrate)

Substrates	Comment Substrates	Organism	Products	Comment (Products)	Rev.	Reac.
D-alanine + H2O + O2	-	Sus scrofa	pyruvic acid + NH3 + H2O2	-	?
D-methionine + H2O + O2	-	Sus scrofa	4-methylthio-2-oxobutanoic acid + NH3 + H2O2	-	?
D-phenylalanine + H2O + O2	-	Sus scrofa	phenylpyruvic acid + NH3 + H2O2	-	?
D-proline + H2O + O2	-	Sus scrofa	2-oxopentanoic acid + NH3 + H2O2	-	?

Turnover Number [1/s]

Turnover Number Minimum [1/s]	Turnover Number Maximum [1/s]	Substrate	Comment	Organism	Structure
5	-	D-alanine	mutant Y228F	Sus scrofa
7.1	-	D-phenylalanine	mutant Y228F	Sus scrofa
11.6	-	D-alanine	mutant Y224F	Sus scrofa
11.7	-	D-proline	mutant Y228F	Sus scrofa
12.7	-	D-alanine	wild-type	Sus scrofa
47.5	-	D-methionine	mutant Y228F	Sus scrofa
48.8	-	D-phenylalanine	mutant Y224F	Sus scrofa
55.9	-	D-phenylalanine	wild-type	Sus scrofa
83.7	-	D-proline	mutant Y224F	Sus scrofa
87.2	-	D-proline	wild-type	Sus scrofa
92	-	D-methionine	mutant Y224F	Sus scrofa
143	-	D-methionine	wild-type	Sus scrofa

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Release 2023.1 (January 2023)