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Literature summary for 1.4.3.3 extracted from

  • Geueke, B.; Weckbecker, A.; Hummel, W.
    Overproduction and characterization of a recombinant D-amino acid oxidase from Arthrobacter protophormiae (2007), Appl. Microbiol. Biotechnol., 74, 1240-1247.
    View publication on PubMed
Search for more literature for 1.4.3.3

Activating Compound

Activating Compound Comment Organism Structure
additional information the activity in the crude extract is not increased when external FAD is added Glutamicibacter protophormiae

Cloned(Commentary)

Cloned (Comment) Organism
expressed in Escherichia coli BL21(DE3) cells Glutamicibacter protophormiae

Inhibitors

Inhibitors Comment Organism Structure
L-methionine slightly inhibited in the presence of L-methionine Glutamicibacter protophormiae

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
34500
-
 2 * 34500, native enzyme, gel filtration Glutamicibacter protophormiae
69000
-
 native enzyme, gel filtration Glutamicibacter protophormiae

Organism

Organism UniProt Comment Textmining
Glutamicibacter protophormiae Q7X2D3
-
-

Purification (Commentary)

Purification (Comment) Organism
Q-Sepharose column chromatography and hydroxyapatite column chromatography Glutamicibacter protophormiae

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
30
-
 recombinant enzyme from crude extract, for D-methionine, at 30°C Glutamicibacter protophormiae
180
-
 recombinant enzyme after 6fold purification, for D-methionine, at 30°C Glutamicibacter protophormiae

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
4-fluoro-DL-phenylalanine + H2O + O2 81% activity compared to D-methionine Glutamicibacter protophormiae 4-fluoro-phenylpyruvate + NH3 + H2O2
-
 ?
D-alanine + H2O + O2 0.26% activity compared to D-methionine Glutamicibacter protophormiae pyruvate + NH3 + H2O2
-
 ?
D-allo-isoleucine + H2O + O2 1.0% activity compared to D-methionine Glutamicibacter protophormiae ?
-
 ?
D-arginine + H2O + O2 80% activity compared to D-methionine Glutamicibacter protophormiae 5-guanidino-2-oxopentanoic acid + NH3 + H2O2
-
 ?
D-histidine + H2O + O2 5.9% activity compared to D-methionine Glutamicibacter protophormiae 3-(1H-imidazol-4-yl)-2-oxopropanoate + NH3 + H2O2
-
 ?
D-leucine + H2O + O2 12% activity compared to D-methionine Glutamicibacter protophormiae 4-methyl-2-oxopentanoic acid + NH3 + H2O2
-
 ?
D-lysine + H2O + O2 99% activity compared to D-methionine Glutamicibacter protophormiae 6-amino-2-oxohexanoic acid + NH3 + H2O2
-
 ?
D-methionine + H2O + O2 100% activity Glutamicibacter protophormiae 4-methylthio-2-oxobutanoic acid + NH3 + H2O2
-
 ?
D-ornithine + H2O + O2 15% activity compared to D-methionine Glutamicibacter protophormiae 5-amino-2-oxopentanoate + NH3 + H2O2
-
 ?
D-phenylalanine + H2O + O2 54% activity compared to D-methionine Glutamicibacter protophormiae phenylpyruvate + NH3 + H2O2
-
 ?
D-phenylalanine + H2O + O2 80% activity compared to D-methionine Glutamicibacter protophormiae phenylpyruvate + NH3 + H2O2
-
 ?
D-proline + H2O + O2 0.25% activity compared to D-methionine Glutamicibacter protophormiae 2-oxopentanoic acid + NH3 + H2O2
-
 ?
D-tyrosine + H2O + O2 4.7% activity compared to D-methionine Glutamicibacter protophormiae 4-hydroxyphenylpyruvic acid + NH3 + H2O2
-
 ?
D-valine + H2O + O2 0.35% activity compared to D-methionine Glutamicibacter protophormiae alpha-ketoisovaleric acid + NH3 + H2O2
-
 ?
DL-homophenylalanine + H2O + O2 31% activity compared to D-methionine Glutamicibacter protophormiae homophenylpyruvate + NH3 + H2O2
-
 ?
DL-tert-leucine + H2O + O2 0.12% activity compared to D-methionine Glutamicibacter protophormiae ?
-
 ?
additional information no activity with glycine, D-serine, D-asparagine, D-aspartate, D-glutamate, D-threonine, D-tryptophan, and cephalosporin C Glutamicibacter protophormiae ?
-
 ?
N-methyl-DL-leucine + H2O + O2 2.1% activity compared to D-methionine Glutamicibacter protophormiae ?
-
 ?
O-methyl-DL-serine + H2O + O2 0.33% activity compared to D-methionine Glutamicibacter protophormiae ?
-
 ?

Subunits

Subunits Comment Organism
homodimer 2 * 34500, native enzyme, gel filtration Glutamicibacter protophormiae

Synonyms

Synonyms Comment Organism
D-AAO
-
 Glutamicibacter protophormiae
D-amino acid oxidase
-
 Glutamicibacter protophormiae

Temperature Stability [°C]

Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
30 50 does not lose activity at temperatures up to 30°C for at least 2 h and has a half-life of 21 min at 40°C and of 2 min at 50°C when it is incubated in 100 mM TEA-HCl buffer (pH 7.6) Glutamicibacter protophormiae

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
6.5 8.5
-
 Glutamicibacter protophormiae

pH Stability

pH Stability pH Stability Maximum Comment Organism
6 9
-
 Glutamicibacter protophormiae

Cofactor

Cofactor Comment Organism Structure
FAD
-
 Glutamicibacter protophormiae

Ki Value [mM]

Ki Value [mM] Ki Value maximum [mM] Inhibitor Comment Organism Structure
315
-
 L-methionine at 30°C Glutamicibacter protophormiae

pI Value

Organism Comment pI Value Maximum pI Value
Glutamicibacter protophormiae isoelectric focusing
-
 4.4