Any feedback?
Please rate this page
(literature.php)
(0/150)

BRENDA support

Literature summary for 1.4.3.3 extracted from

  • Hsieh, H.C.; Kuan, I.C.; Lee, S.L.; Tien, G.Y.; Wang, Y.J.; Yu, C.Y.
    Stabilization of D-amino acid oxidase from Rhodosporidium toruloides by immobilization onto magnetic nanoparticles (2009), Biotechnol. Lett., 31, 557-563.
    View publication on PubMed

Protein Variants

Protein Variants Comment Organism
additional information immobilization of the enzyme onto magnetic nanoparticles stabilizes the enzyme Rhodotorula toruloides

General Stability

General Stability Organism
stabilization of the enzyme by immobilization onto Fe3O4 magnetic nanoparticles, after immobilization the Tm increases from 45°C of the free form to 55°C. In the presence of 20 mM H2O2, the immobilized form retains 93% of its activity after 5 h while the free form is completely inactivated after 3.5 h Rhodotorula toruloides

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.7
-
D-alanine free enzyme Rhodotorula toruloides
1
-
D-alanine immobilized enzyme Rhodotorula toruloides

Organism

Organism UniProt Comment Textmining
Rhodotorula toruloides P80324
-
-

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
27
-
immobiliuzed enzyme Rhodotorula toruloides
59
-
free enzyme Rhodotorula toruloides

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
D-alanine + H2O + O2
-
Rhodotorula toruloides pyruvate + NH3 + H2O2
-
?

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
40
-
free enzyme Rhodotorula toruloides
50
-
immobilized enzyme Rhodotorula toruloides

Turnover Number [1/s]

Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
13.8
-
D-alanine immobilized enzyme Rhodotorula toruloides
33.8
-
D-alanine free enzyme Rhodotorula toruloides

pH Stability

pH Stability pH Stability Maximum Comment Organism
5 10 free and immobilized enzymes, stable, profile, overview Rhodotorula toruloides

kcat/KM [mM/s]

kcat/KM Value [1/mMs-1] kcat/KM Value Maximum [1/mMs-1] Substrate Comment Organism Structure
13.8
-
D-alanine immobilized enzyme Rhodotorula toruloides
49.7
-
D-alanine free enzyme Rhodotorula toruloides