Any feedback?
Please rate this page
(literature.php)
(0/150)

BRENDA support

Literature summary for 1.4.3.3 extracted from

  • Abad, S.; Nahalka, J.; Bergler, G.; Arnold, S.A.; Speight, R.; Fotheringham, I.; Nidetzky, B.; Glieder, A.
    Stepwise engineering of a Pichia pastoris D-amino acid oxidase whole cell catalyst (2010), Microb. Cell Fact., 9, 24.
    View publication on PubMedView publication on EuropePMC

Application

Application Comment Organism
biotechnology the enzyme is used as a biocatalyst in cephalosporin C conversion on industrial scale Trigonopsis variabilis

Cloned(Commentary)

Cloned (Comment) Organism
stable expression in Pichia pastoris with 7fold enhancement of the intracellular level of oxidase activity, expression optimization by codon redesign as well as efficient subcellular targeting of the enzyme to peroxisomes Trigonopsis variabilis

Protein Variants

Protein Variants Comment Organism
additional information development of a robust and highly active Pichia pastoris TvDAO whole-cell biocatalyst in a multistep engineering process for use in cephalosporin C conversion on industrial scale, overview. Usage of a fed-batch cultivation of the multicopy strain Trigonopsis variabilis

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
D-amino acid + H2O + O2 Trigonopsis variabilis
-
2-oxo acid + NH3 + H2O2
-
?

Organism

Organism UniProt Comment Textmining
Trigonopsis variabilis Q6R4Q9
-
-

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
additional information
-
intracellular activity of recombinant DAO in transformed Pichia pastoris, overview Trigonopsis variabilis

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
D-amino acid + H2O + O2
-
Trigonopsis variabilis 2-oxo acid + NH3 + H2O2
-
?
D-amino acid + H2O + O2 via formation of an imino acid Trigonopsis variabilis 2-oxo acid + NH3 + H2O2
-
?
additional information conversion of cephalosporine C to 7-aminocephalosporanic acid and spontaneous decarboxylation of oxoadipyl-7-amino cephalosporanic acid is promoted by the H2O2 formed in the oxidase reaction of TvDAO Trigonopsis variabilis ?
-
?

Synonyms

Synonyms Comment Organism
DAO
-
Trigonopsis variabilis