Cloned (Comment) | Organism |
---|---|
expressed in Pichia pastoris | Rattus norvegicus |
overexpression of MAO-A in Pichia pastoris strain KM71 | Rattus norvegicus |
General Stability | Organism |
---|---|
50% glycerol stabilize the enzyme | Rattus norvegicus |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
Clorgyline | MAO-A forms a N(5) flavocyanine adduct on inhibition by clorgyline, MAO A is blocked at an N-terminal threonyl residue | Rattus norvegicus | |
D-amphetamine | reversible inhibitor; reversible inhibitor | Rattus norvegicus | |
isatin | reversible inhibitor; reversible inhibitor | Rattus norvegicus | |
additional information | no inhibition of MAO-A by farnesol, chlorostyrylcaffeine, and 1,4-diphenyl-2-butene; not inhibited by farnesol, chlorostyrylcaffeine and 1,4-diphenyl-2-butene | Rattus norvegicus | |
phentermine | reversible inhibitor; reversible inhibitor | Rattus norvegicus |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
additional information | - |
additional information | steady-state kinetic studies, recombinant enzyme, overview | Rattus norvegicus | |
0.0093 | - |
O2 | isoform MAO A, in 50 mM potassium phosphate assay buffer (pH 7.5), at 25°C | Rattus norvegicus | |
0.09 | - |
kynuramine | pH 7.5, 25°C, MAO-A | Rattus norvegicus | |
0.09 | - |
kynuramine | isoform MAO A, in 50 mM potassium phosphate assay buffer (pH 7.5), at 25°C | Rattus norvegicus | |
0.21 | - |
benzylamine | pH 7.5, 25°C, MAO-A | Rattus norvegicus | |
0.21 | - |
benzylamine | isoform MAO A, in 50 mM potassium phosphate assay buffer (pH 7.5), at 25°C | Rattus norvegicus | |
0.3 | - |
serotonin | pH 7.5, 25°C, MAO-A | Rattus norvegicus | |
0.3 | - |
serotonin | isoform MAO A, in 50 mM potassium phosphate assay buffer (pH 7.5), at 25°C | Rattus norvegicus | |
0.35 | - |
Phenylethylamine | pH 7.5, 25°C, MAO-A | Rattus norvegicus | |
0.35 | - |
2-Phenylethylamine | isoform MAO A, in 50 mM potassium phosphate assay buffer (pH 7.5), at 25°C | Rattus norvegicus |
Localization | Comment | Organism | GeneOntology No. | Textmining |
---|---|---|---|---|
mitochondrial membrane | outer | Rattus norvegicus | 31966 | - |
mitochondrial outer membrane | - |
Rattus norvegicus | 5741 | - |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
60000 | - |
x * 60000, recombinant MAO-A, SDS-PAGE | Rattus norvegicus |
60000 | - |
x * 60000, isoform MAO A, SDS-PAGE | Rattus norvegicus |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
benzylamine + H2O + O2 | Rattus norvegicus | MAO-A | benzaldehyde + NH3 + H2O2 | - |
? | |
kynuramine + H2O + O2 | Rattus norvegicus | MAO-A | 3-(2-aminophenyl)-3-oxopropanal + NH3 + H2O2 | - |
? | |
phenylethylamine + H2O + O2 | Rattus norvegicus | MAO-A | 2-phenylethanal + NH3 + H2O2 | - |
? | |
serotonin + H2O + O2 | Rattus norvegicus | MAO-A | (5-hydroxy-1H-indol-3-yl)acetaldehyde + NH3 + H2O2 | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Rattus norvegicus | - |
- |
- |
Rattus norvegicus | P21396 | - |
- |
Posttranslational Modification | Comment | Organism |
---|---|---|
flavoprotein | - |
Rattus norvegicus |
Purification (Comment) | Organism |
---|---|
DEAE-Sepharose column chromatography | Rattus norvegicus |
recombinant MAO-A from Pichia pastoris strain KM71 outer mitochondrial membranes by Triton X-100 solubilization and anion exchange chromatography | Rattus norvegicus |
Source Tissue | Comment | Organism | Textmining |
---|---|---|---|
liver | - |
Rattus norvegicus | - |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
0.025 | - |
cell lysate, in 50 mM potassium phosphate assay buffer (pH 7.5), at 25°C | Rattus norvegicus |
1.42 | - |
purified recombinant MAO-A | Rattus norvegicus |
1.42 | - |
after purification, isoform MAO A, in 50 mM potassium phosphate assay buffer (pH 7.5), at 25°C | Rattus norvegicus |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
2-phenylethylamine + H2O + O2 | - |
Rattus norvegicus | 2-phenylethanal + NH3 + H2O2 | - |
? | |
benzylamine + H2O + O2 | - |
Rattus norvegicus | benzaldehyde + NH3 + H2O2 | - |
? | |
benzylamine + H2O + O2 | MAO-A | Rattus norvegicus | benzaldehyde + NH3 + H2O2 | - |
? | |
kynuramine + H2O + O2 | - |
Rattus norvegicus | 3-(2-aminophenyl)-3-oxopropanal + NH3 + H2O2 | - |
? | |
kynuramine + H2O + O2 | MAO-A | Rattus norvegicus | 3-(2-aminophenyl)-3-oxopropanal + NH3 + H2O2 | - |
? | |
phenylethylamine + H2O + O2 | MAO-A | Rattus norvegicus | 2-phenylethanal + NH3 + H2O2 | - |
? | |
serotonin + H2O + O2 | - |
Rattus norvegicus | (5-hydroxy-1H-indol-3-yl)acetaldehyde + NH3 + H2O2 | - |
? | |
serotonin + H2O + O2 | MAO-A | Rattus norvegicus | (5-hydroxy-1H-indol-3-yl)acetaldehyde + NH3 + H2O2 | - |
? |
Subunits | Comment | Organism |
---|---|---|
? | x * 60000, recombinant MAO-A, SDS-PAGE | Rattus norvegicus |
? | x * 60000, isoform MAO A, SDS-PAGE | Rattus norvegicus |
More | peptide mapping, MALDI-TOF spectroscopic analysis, overview | Rattus norvegicus |
Synonyms | Comment | Organism |
---|---|---|
MAO A | - |
Rattus norvegicus |
MAO A | isoform | Rattus norvegicus |
monoamine oxidase A | - |
Rattus norvegicus |
monoamine oxidase A | isoform | Rattus norvegicus |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
25 | - |
assay at | Rattus norvegicus |
Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
---|---|---|---|
- |
30 | isoform MAO A remains fully active when incubated at 0°C for 120 minutes. At 25°C, only 10% of isoform MAO A activity is lost after 120 min incubation. The enzyme retains about 80% catalytic activity after incubation at 30°C for 120 min | Rattus norvegicus |
25 | - |
purified recombinant MAO-A, 120 min, loss of 10% activity | Rattus norvegicus |
30 | - |
purified recombinant MAO-A, 120 min, loss of 20% activity | Rattus norvegicus |
Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.325 | - |
benzylamine | pH 7.5, 25°C, MAO-A | Rattus norvegicus | |
0.325 | - |
benzylamine | isoform MAO A, in 50 mM potassium phosphate assay buffer (pH 7.5), at 25°C | Rattus norvegicus | |
1.42 | - |
Phenylethylamine | pH 7.5, 25°C, MAO-A | Rattus norvegicus | |
1.42 | - |
2-Phenylethylamine | isoform MAO A, in 50 mM potassium phosphate assay buffer (pH 7.5), at 25°C | Rattus norvegicus | |
2.48 | - |
kynuramine | isoform MAO A, in 50 mM potassium phosphate assay buffer (pH 7.5), at 25°C | Rattus norvegicus | |
2.485 | - |
kynuramine | pH 7.5, 25°C, MAO-A | Rattus norvegicus | |
5.98 | - |
serotonin | pH 7.5, 25°C, MAO-A | Rattus norvegicus | |
5.98 | - |
serotonin | isoform MAO A, in 50 mM potassium phosphate assay buffer (pH 7.5), at 25°C | Rattus norvegicus |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
7.5 | - |
assay at | Rattus norvegicus |
Cofactor | Comment | Organism | Structure |
---|---|---|---|
FAD | covalently bound | Rattus norvegicus | |
FAD | the enzyme contains a covalently bound FAD | Rattus norvegicus |
Ki Value [mM] | Ki Value maximum [mM] | Inhibitor | Comment | Organism | Structure |
---|---|---|---|---|---|
0.006 | - |
D-amphetamine | pH 7.5, 25°C, MAO-A | Rattus norvegicus | |
0.0105 | - |
isatin | pH 7.5, 25°C, MAO-A | Rattus norvegicus | |
0.626 | - |
phentermine | pH 7.5, 25°C, MAO-A | Rattus norvegicus | |
6 | - |
D-amphetamine | isoform MAO A, in 50 mM potassium phosphate assay buffer (pH 7.5), at 25°C | Rattus norvegicus | |
10 | - |
isatin | isoform MAO A, in 50 mM potassium phosphate assay buffer (pH 7.5), at 25°C | Rattus norvegicus | |
625 | - |
phentermine | isoform MAO A, in 50 mM potassium phosphate assay buffer (pH 7.5), at 25°C | Rattus norvegicus |
kcat/KM Value [1/mMs-1] | kcat/KM Value Maximum [1/mMs-1] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
1.55 | - |
benzylamine | pH 7.5, 25°C, MAO-A | Rattus norvegicus | |
1.55 | - |
benzylamine | isoform MAO A, in 50 mM potassium phosphate assay buffer (pH 7.5), at 25°C | Rattus norvegicus | |
4.05 | - |
Phenylethylamine | pH 7.5, 25°C, MAO-A | Rattus norvegicus | |
4.05 | - |
2-Phenylethylamine | isoform MAO A, in 50 mM potassium phosphate assay buffer (pH 7.5), at 25°C | Rattus norvegicus | |
19.93 | - |
serotonin | pH 7.5, 25°C, MAO-A | Rattus norvegicus | |
19.93 | - |
serotonin | isoform MAO A, in 50 mM potassium phosphate assay buffer (pH 7.5), at 25°C | Rattus norvegicus | |
27.61 | - |
kynuramine | pH 7.5, 25°C, MAO-A | Rattus norvegicus | |
27.61 | - |
kynuramine | isoform MAO A, in 50 mM potassium phosphate assay buffer (pH 7.5), at 25°C | Rattus norvegicus |