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Literature summary for 1.4.3.4 extracted from

  • Wang, J.; Edmondson, D.E.
    High-level expression and purification of rat monoamine oxidase A (MAO A) in Pichia pastoris: comparison with human MAO A (2009), Protein Expr. Purif., 70, 211-217.
    View publication on PubMedView publication on EuropePMC

Cloned(Commentary)

Cloned (Comment) Organism
expressed in Pichia pastoris Rattus norvegicus
overexpression of MAO-A in Pichia pastoris strain KM71 Rattus norvegicus

General Stability

General Stability Organism
50% glycerol stabilize the enzyme Rattus norvegicus

Inhibitors

Inhibitors Comment Organism Structure
Clorgyline MAO-A forms a N(5) flavocyanine adduct on inhibition by clorgyline, MAO A is blocked at an N-terminal threonyl residue Rattus norvegicus
D-amphetamine reversible inhibitor; reversible inhibitor Rattus norvegicus
isatin reversible inhibitor; reversible inhibitor Rattus norvegicus
additional information no inhibition of MAO-A by farnesol, chlorostyrylcaffeine, and 1,4-diphenyl-2-butene; not inhibited by farnesol, chlorostyrylcaffeine and 1,4-diphenyl-2-butene Rattus norvegicus
phentermine reversible inhibitor; reversible inhibitor Rattus norvegicus

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
additional information
-
additional information steady-state kinetic studies, recombinant enzyme, overview Rattus norvegicus
0.0093
-
O2 isoform MAO A, in 50 mM potassium phosphate assay buffer (pH 7.5), at 25°C Rattus norvegicus
0.09
-
kynuramine pH 7.5, 25°C, MAO-A Rattus norvegicus
0.09
-
kynuramine isoform MAO A, in 50 mM potassium phosphate assay buffer (pH 7.5), at 25°C Rattus norvegicus
0.21
-
benzylamine pH 7.5, 25°C, MAO-A Rattus norvegicus
0.21
-
benzylamine isoform MAO A, in 50 mM potassium phosphate assay buffer (pH 7.5), at 25°C Rattus norvegicus
0.3
-
serotonin pH 7.5, 25°C, MAO-A Rattus norvegicus
0.3
-
serotonin isoform MAO A, in 50 mM potassium phosphate assay buffer (pH 7.5), at 25°C Rattus norvegicus
0.35
-
Phenylethylamine pH 7.5, 25°C, MAO-A Rattus norvegicus
0.35
-
2-Phenylethylamine isoform MAO A, in 50 mM potassium phosphate assay buffer (pH 7.5), at 25°C Rattus norvegicus

Localization

Localization Comment Organism GeneOntology No. Textmining
mitochondrial membrane outer Rattus norvegicus 31966
-
mitochondrial outer membrane
-
Rattus norvegicus 5741
-

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
60000
-
x * 60000, recombinant MAO-A, SDS-PAGE Rattus norvegicus
60000
-
x * 60000, isoform MAO A, SDS-PAGE Rattus norvegicus

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
benzylamine + H2O + O2 Rattus norvegicus MAO-A benzaldehyde + NH3 + H2O2
-
?
kynuramine + H2O + O2 Rattus norvegicus MAO-A 3-(2-aminophenyl)-3-oxopropanal + NH3 + H2O2
-
?
phenylethylamine + H2O + O2 Rattus norvegicus MAO-A 2-phenylethanal + NH3 + H2O2
-
?
serotonin + H2O + O2 Rattus norvegicus MAO-A (5-hydroxy-1H-indol-3-yl)acetaldehyde + NH3 + H2O2
-
?

Organism

Organism UniProt Comment Textmining
Rattus norvegicus
-
-
-
Rattus norvegicus P21396
-
-

Posttranslational Modification

Posttranslational Modification Comment Organism
flavoprotein
-
Rattus norvegicus

Purification (Commentary)

Purification (Comment) Organism
DEAE-Sepharose column chromatography Rattus norvegicus
recombinant MAO-A from Pichia pastoris strain KM71 outer mitochondrial membranes by Triton X-100 solubilization and anion exchange chromatography Rattus norvegicus

Source Tissue

Source Tissue Comment Organism Textmining
liver
-
Rattus norvegicus
-

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
0.025
-
cell lysate, in 50 mM potassium phosphate assay buffer (pH 7.5), at 25°C Rattus norvegicus
1.42
-
purified recombinant MAO-A Rattus norvegicus
1.42
-
after purification, isoform MAO A, in 50 mM potassium phosphate assay buffer (pH 7.5), at 25°C Rattus norvegicus

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2-phenylethylamine + H2O + O2
-
Rattus norvegicus 2-phenylethanal + NH3 + H2O2
-
?
benzylamine + H2O + O2
-
Rattus norvegicus benzaldehyde + NH3 + H2O2
-
?
benzylamine + H2O + O2 MAO-A Rattus norvegicus benzaldehyde + NH3 + H2O2
-
?
kynuramine + H2O + O2
-
Rattus norvegicus 3-(2-aminophenyl)-3-oxopropanal + NH3 + H2O2
-
?
kynuramine + H2O + O2 MAO-A Rattus norvegicus 3-(2-aminophenyl)-3-oxopropanal + NH3 + H2O2
-
?
phenylethylamine + H2O + O2 MAO-A Rattus norvegicus 2-phenylethanal + NH3 + H2O2
-
?
serotonin + H2O + O2
-
Rattus norvegicus (5-hydroxy-1H-indol-3-yl)acetaldehyde + NH3 + H2O2
-
?
serotonin + H2O + O2 MAO-A Rattus norvegicus (5-hydroxy-1H-indol-3-yl)acetaldehyde + NH3 + H2O2
-
?

Subunits

Subunits Comment Organism
? x * 60000, recombinant MAO-A, SDS-PAGE Rattus norvegicus
? x * 60000, isoform MAO A, SDS-PAGE Rattus norvegicus
More peptide mapping, MALDI-TOF spectroscopic analysis, overview Rattus norvegicus

Synonyms

Synonyms Comment Organism
MAO A
-
Rattus norvegicus
MAO A isoform Rattus norvegicus
monoamine oxidase A
-
Rattus norvegicus
monoamine oxidase A isoform Rattus norvegicus

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
25
-
assay at Rattus norvegicus

Temperature Stability [°C]

Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
-
30 isoform MAO A remains fully active when incubated at 0°C for 120 minutes. At 25°C, only 10% of isoform MAO A activity is lost after 120 min incubation. The enzyme retains about 80% catalytic activity after incubation at 30°C for 120 min Rattus norvegicus
25
-
purified recombinant MAO-A, 120 min, loss of 10% activity Rattus norvegicus
30
-
purified recombinant MAO-A, 120 min, loss of 20% activity Rattus norvegicus

Turnover Number [1/s]

Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
0.325
-
benzylamine pH 7.5, 25°C, MAO-A Rattus norvegicus
0.325
-
benzylamine isoform MAO A, in 50 mM potassium phosphate assay buffer (pH 7.5), at 25°C Rattus norvegicus
1.42
-
Phenylethylamine pH 7.5, 25°C, MAO-A Rattus norvegicus
1.42
-
2-Phenylethylamine isoform MAO A, in 50 mM potassium phosphate assay buffer (pH 7.5), at 25°C Rattus norvegicus
2.48
-
kynuramine isoform MAO A, in 50 mM potassium phosphate assay buffer (pH 7.5), at 25°C Rattus norvegicus
2.485
-
kynuramine pH 7.5, 25°C, MAO-A Rattus norvegicus
5.98
-
serotonin pH 7.5, 25°C, MAO-A Rattus norvegicus
5.98
-
serotonin isoform MAO A, in 50 mM potassium phosphate assay buffer (pH 7.5), at 25°C Rattus norvegicus

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
7.5
-
assay at Rattus norvegicus

Cofactor

Cofactor Comment Organism Structure
FAD covalently bound Rattus norvegicus
FAD the enzyme contains a covalently bound FAD Rattus norvegicus

Ki Value [mM]

Ki Value [mM] Ki Value maximum [mM] Inhibitor Comment Organism Structure
0.006
-
D-amphetamine pH 7.5, 25°C, MAO-A Rattus norvegicus
0.0105
-
isatin pH 7.5, 25°C, MAO-A Rattus norvegicus
0.626
-
phentermine pH 7.5, 25°C, MAO-A Rattus norvegicus
6
-
D-amphetamine isoform MAO A, in 50 mM potassium phosphate assay buffer (pH 7.5), at 25°C Rattus norvegicus
10
-
isatin isoform MAO A, in 50 mM potassium phosphate assay buffer (pH 7.5), at 25°C Rattus norvegicus
625
-
phentermine isoform MAO A, in 50 mM potassium phosphate assay buffer (pH 7.5), at 25°C Rattus norvegicus

kcat/KM [mM/s]

kcat/KM Value [1/mMs-1] kcat/KM Value Maximum [1/mMs-1] Substrate Comment Organism Structure
1.55
-
benzylamine pH 7.5, 25°C, MAO-A Rattus norvegicus
1.55
-
benzylamine isoform MAO A, in 50 mM potassium phosphate assay buffer (pH 7.5), at 25°C Rattus norvegicus
4.05
-
Phenylethylamine pH 7.5, 25°C, MAO-A Rattus norvegicus
4.05
-
2-Phenylethylamine isoform MAO A, in 50 mM potassium phosphate assay buffer (pH 7.5), at 25°C Rattus norvegicus
19.93
-
serotonin pH 7.5, 25°C, MAO-A Rattus norvegicus
19.93
-
serotonin isoform MAO A, in 50 mM potassium phosphate assay buffer (pH 7.5), at 25°C Rattus norvegicus
27.61
-
kynuramine pH 7.5, 25°C, MAO-A Rattus norvegicus
27.61
-
kynuramine isoform MAO A, in 50 mM potassium phosphate assay buffer (pH 7.5), at 25°C Rattus norvegicus