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Literature summary for 1.4.3.5 extracted from

  • Cash, C.D.; Maitre, M.; Rumigny, J.F.; Mandel, P.
    Rapid purification by affinity chromatography of rat brain pyridoxal kinase and pyridoxamine-5-phosphate oxidase (1980), Biochem. Biophys. Res. Commun., 96, 1755-1760.
    View publication on PubMed
Search for more literature for 1.4.3.5

Organism

Organism UniProt Comment Textmining
Rattus norvegicus
-
-
-

Purification (Commentary)

Purification (Comment) Organism
affinity chromatography Rattus norvegicus
native enzyme 3175fold from brain by pyridoxyl and phosphopyridoxyl affinity chromatography, gel filtration, and anion exchange chromatography, to homogeneity Rattus norvegicus

Source Tissue

Source Tissue Comment Organism Textmining
brain
-
 Rattus norvegicus
-

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
additional information
-
 purified enzyme Rattus norvegicus
1.708
-
-
 Rattus norvegicus

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
pyridoxamine 5'-phosphate + FMN + H2O + 2 H+
-
 Rattus norvegicus pyridoxal 5'-phosphate + FMNH2 + NH3
-
 ?
pyridoxamine 5'-phosphate + H2O + O2
-
 Rattus norvegicus pyridoxal 5'-phosphate + NH3 + H2O2
-
 ?
pyridoxine 5'-phosphate + FMN
-
 Rattus norvegicus pyridoxal 5'-phosphate + FMNH2
-
 ?

Synonyms

Synonyms Comment Organism
pyridoxamine 5'-phosphate oxidase
-
 Rattus norvegicus
pyridoxamine-5-phosphate oxidase
-
 Rattus norvegicus

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
37
-
 assay at Rattus norvegicus

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
11
-
 assay at Rattus norvegicus

Cofactor

Cofactor Comment Organism Structure
FMN
-
 Rattus norvegicus