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Literature summary for 1.4.7.1 extracted from

  • Hirasawa, M.; Tamura, G.
    Flavin and iron-sulfur containing ferredoxin-linked glutamate synthase from spinach leaves (1984), J. Biochem., 95, 983-994.
    View publication on PubMed
Search for more literature for 1.4.7.1

Inhibitors

Inhibitors Comment Organism Structure
Atebrin 1 mM, 45% inhibition Spinacia oleracea
azaserine 1 mM, complete inhibition Spinacia oleracea
p-chloromercuribenzoate 0.1 mM, complete inhibition Spinacia oleracea
p-chloromercuriphenylsulfonate 0.1 mM, complete inhibition Spinacia oleracea

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.062
-
 2-oxoglutarate
-
 Spinacia oleracea
0.53
-
 L-glutamine
-
 Spinacia oleracea

Metals/Ions

Metals/Ions Comment Organism Structure
Iron enzyme contains an iron-sulfur-center Spinacia oleracea

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
140000
-
 gel filtration Spinacia oleracea
170000
-
 1 * 170000, SDS-PAGE Spinacia oleracea

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
L-glutamine + 2-oxoglutarate + reduced ferredoxin Spinacia oleracea
-
 L-glutamate + oxidized ferredoxin
-
 ?

Organism

Organism UniProt Comment Textmining
Spinacia oleracea
-
 spinach
-

Purification (Commentary)

Purification (Comment) Organism
affinity chromatography on ferredoxin Sepharose Spinacia oleracea

Source Tissue

Source Tissue Comment Organism Textmining
leaf
-
 Spinacia oleracea
-

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
101.7
-
-
 Spinacia oleracea

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
L-glutamine + 2-oxoglutarate + reduced ferredoxin
-
 Spinacia oleracea L-glutamate + oxidized ferredoxin
-
 ?
L-glutamine + 2-oxoglutarate + reduced methylviologen only in the presence of ferredoxin Spinacia oleracea L-glutamate + oxidized methylviologen
-
 ?

Subunits

Subunits Comment Organism
monomer 1 * 170000, SDS-PAGE Spinacia oleracea

Cofactor

Cofactor Comment Organism Structure
FMN
-
 Spinacia oleracea