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Literature summary for 1.4.7.1 extracted from

  • Kameya, M.; Ikeda, T.; Nakamura, M.; Arai, H.; Ishii, M.; Igarashi, Y.
    A novel ferredoxin-dependent glutamate synthase from the hydrogen-oxidizing chemoautotrophic bacterium, Hydrogenobacter thermophilus TK-6 (2007), J. Bacteriol., 189, 2805-2812.
    View publication on PubMedView publication on EuropePMC

Activating Compound

Activating Compound Comment Organism Structure
citrate up to 50% increase in activity Hydrogenobacter thermophilus
malate up to 50% increase in activity Hydrogenobacter thermophilus
oxaloacetate up to 50% increase in activity Hydrogenobacter thermophilus
succinate up to 50% increase in activity Hydrogenobacter thermophilus

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.005
-
reduced bacterial ferredoxin 1 in 20 mM NaPO4 (pH 7.2), at 70°C Hydrogenobacter thermophilus
0.28
-
2-oxoglutarate pH 8.0, 70°C Hydrogenobacter thermophilus
0.28
-
2-oxoglutarate in 20 mM NaPO4 (pH 7.2), 10 mM L-glutamine, 5 mM methylviologen, 5 mM dithionite, at 70°C Hydrogenobacter thermophilus
1.1
-
L-glutamine pH 8.0, 70°C Hydrogenobacter thermophilus
1.1
-
L-glutamine in 20 mM NaPO4 (pH 7.2), 1 mM 2-oxoglutarate, 5 mM methylviologen, 5 mM dithionite, at 70°C Hydrogenobacter thermophilus

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
115000
-
gel filtration Hydrogenobacter thermophilus
151000
-
SDS-PAGE Hydrogenobacter thermophilus
151000
-
1 * 151000, SDS-PAGE Hydrogenobacter thermophilus
168000
-
calculated from amino acid sequence Hydrogenobacter thermophilus

Organism

Organism UniProt Comment Textmining
Hydrogenobacter thermophilus
-
-
-
Hydrogenobacter thermophilus A1IJT7
-
-
Hydrogenobacter thermophilus TK-6 / IAM 12695
-
-
-
Hydrogenobacter thermophilus TK-6 / IAM 12695 A1IJT7
-
-

Purification (Commentary)

Purification (Comment) Organism
ammonium sulfate precipitation, hydroxyapatite column chromatography, butyl-Toyopearl column chromatography, MonoQ column chromatography, and DEAE-Toyopearl column chromatography Hydrogenobacter thermophilus

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
0.0078
-
crude extract, at 70°C Hydrogenobacter thermophilus
0.6
-
pH 8.0, 70°C Hydrogenobacter thermophilus
1.5
-
after 191fold purification, at 70°C Hydrogenobacter thermophilus

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
L-glutamine + 2-oxoglutarate + reduced bacterial ferredoxin 1
-
Hydrogenobacter thermophilus L-glutamate + oxidized bacterial ferredoxin 1
-
?
L-glutamine + 2-oxoglutarate + reduced bacterial ferredoxin 1
-
Hydrogenobacter thermophilus TK-6 / IAM 12695 L-glutamate + oxidized bacterial ferredoxin 1
-
?
L-glutamine + 2-oxoglutarate + reduced bacterial ferredoxin 2
-
Hydrogenobacter thermophilus L-glutamate + oxidized bacterial ferredoxin 2
-
?
L-glutamine + 2-oxoglutarate + reduced bacterial ferredoxin 2
-
Hydrogenobacter thermophilus TK-6 / IAM 12695 L-glutamate + oxidized bacterial ferredoxin 2
-
?
L-glutamine + 2-oxoglutarate + reduced methylviologen
-
Hydrogenobacter thermophilus L-glutamate + oxidized methylviologen
-
?
L-glutamine + 2-oxoglutarate + reduced methylviologen
-
Hydrogenobacter thermophilus TK-6 / IAM 12695 L-glutamate + oxidized methylviologen
-
?
additional information does not react with plant ferredoxin 3 Hydrogenobacter thermophilus ?
-
?
additional information does not react with plant ferredoxin 3 Hydrogenobacter thermophilus TK-6 / IAM 12695 ?
-
?

Subunits

Subunits Comment Organism
monomer 1 * 151000, SDS-PAGE Hydrogenobacter thermophilus

Synonyms

Synonyms Comment Organism
Fd-GOGAT
-
Hydrogenobacter thermophilus
ferredoxin-dependent glutamate synthase exhibits also glutaminase activity and does not react with a plant-type ferredoxin (Fd3) containing a [2Fe-2S] cluster, but does react with bacterial ferredoxins (Fd1 and Fd2) containing [4Fe-4S] clusters Hydrogenobacter thermophilus
glutamine:2-oxoglutarate amidotransferase
-
Hydrogenobacter thermophilus

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
70 80
-
Hydrogenobacter thermophilus

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
6.9 7.4 at 70°C Hydrogenobacter thermophilus

Cofactor

Cofactor Comment Organism Structure
Ferredoxin enzyme does not react with plant-type ferredoxin but with a bacterial-type ferredoxin containing [4Fe-4S] clusters Hydrogenobacter thermophilus
FMN 0.9 mol per mol of protein Hydrogenobacter thermophilus