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Literature summary for 1.5.1.11 extracted from

  • Gäde, G.; Carlsson, K.H.
    Purification and characterisation of octopine dehydrogenase from marine nemertean Cerebratulus lactus (anopla: heteronemerta): comparison with scallop octopine dehydrogenase (1984), Mar. Biol., 79, 39-45.
No PubMed abstract available
Search for more literature for 1.5.1.11

Inhibitors

Inhibitors Comment Organism Structure
octopine substrate inhibition above 2 mM, 50% product inhibition at 1.2 mM Cerebratulus lacteus

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.014
-
 NAD+
-
 Cerebratulus lacteus
0.028
-
 NADH
-
 Cerebratulus lacteus
0.22
-
 octopine
-
 Cerebratulus lacteus
0.5 1.5 pyruvate depending on concentration of the cosubstrates NADH and Arg Cerebratulus lacteus
1.4 2.8 Arg dependent on concentration of the cosubstrates NADH and pyruvate Cerebratulus lacteus

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
39000
-
 ultracentrifugal studies Cerebratulus lacteus
41500
-
 1 * 41500, SDS-PAGE Cerebratulus lacteus

Organism

Organism UniProt Comment Textmining
Cerebratulus lacteus
-
-
-
Pecten jacobaeus
-
-
-

Purification (Commentary)

Purification (Comment) Organism
-
 Cerebratulus lacteus

Source Tissue

Source Tissue Comment Organism Textmining
adductor
-
 Pecten jacobaeus
-

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
additional information
-
-
 Cerebratulus lacteus

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
Canavanine + pyruvate + NADH L-canavanine, at 56% of the activity with L-Arg Cerebratulus lacteus N2-(D-1-Carboxyethyl)-canavanine + NAD+ + H2O
-
 ?
Canavanine + pyruvate + NADH L-canavanine, at 48% of the activity with L-Arg Pecten jacobaeus N2-(D-1-Carboxyethyl)-canavanine + NAD+ + H2O
-
 ?
Homoarginine + pyruvate + NADH at 22% of the activity with L-Arg Pecten jacobaeus N2-(D-1-Carboxyethyl)-homoarginine + NAD+ + H2O
-
 ?
Homoarginine + pyruvate + NADH at 13% of the activity with L-Arg Cerebratulus lacteus N2-(D-1-Carboxyethyl)-homoarginine + NAD+ + H2O
-
 ?
L-Arg + 2-oxobutanoate + NADH at 41% of the activity with L-Arg Cerebratulus lacteus N2-(D-2-Carboxypropyl)-L-Arg + NAD+ + H2O
-
 ?
L-Arg + 2-oxobutanoate + NADH at 28% of the activity with pyruvate Pecten jacobaeus N2-(D-2-Carboxypropyl)-L-Arg + NAD+ + H2O
-
 ?
L-Arg + pyruvate + NADH
-
 Pecten jacobaeus N2-(D-1-carboxyethyl)-L-Arg + NAD+ + H2O
-
 ?
L-Arg + pyruvate + NADH r Cerebratulus lacteus N2-(D-1-carboxyethyl)-L-Arg + NAD+ + H2O
-
 ?
L-Lys + pyruvate + NADH at 2% of the activity with L-Arg Pecten jacobaeus Lysopine + NAD+ + H2O
-
 ?
L-Lys + pyruvate + NADH at 1% of the activity with L-Arg Cerebratulus lacteus Lysopine + NAD+ + H2O
-
 ?
Orn + pyruvate + NADH at 2% of the activity with L-Arg Cerebratulus lacteus N2-(D-1-Carboxyethyl)-L-Orn + NAD+ + H2O
-
 ?
Orn + pyruvate + NADH at 4% of the activity with L-Arg Pecten jacobaeus N2-(D-1-Carboxyethyl)-L-Orn + NAD+ + H2O
-
 ?

Subunits

Subunits Comment Organism
monomer 1 * 41500, SDS-PAGE Cerebratulus lacteus

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
7
-
-
 Cerebratulus lacteus

pH Range

pH Minimum pH Maximum Comment Organism
6 8 about 70% of maximal activity at pH 6 and pH 8 Cerebratulus lacteus

Cofactor

Cofactor Comment Organism Structure
NADH
-
 Pecten jacobaeus
NADH
-
 Cerebratulus lacteus