Cloned (Comment) | Organism |
---|---|
expressed in Escherichia coli with a C-terminal penta His-tag | Pecten maximus |
Protein Variants | Comment | Organism |
---|---|---|
C148A | the mutant shows reduced activity | Pecten maximus |
C148S | the mutant shows reduced activity and is only slightly inhibited by 5,5'-dithiobis(2-nitrobenzoic acid) and p-chloromercuribenzoate | Pecten maximus |
D329A | the mutation results in strong increases in Km and decreases in kcat values for pyruvate and L-arginine, but has little effect on the Km and kcat values for NADH | Pecten maximus |
H212A | the mutation results in strong increases in Km and decreases in kcat values for pyruvate and L-arginine, but has little effect on the Km and kcat values for NADH | Pecten maximus |
R324A | the mutation results in strong increases in Km and decreases in kcat values for pyruvate and L-arginine, but has little effect on the Km and kcat values for NADH | Pecten maximus |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
5,5'-dithiobis(2-nitrobenzoic acid) | - |
Pecten maximus | |
additional information | ODH is not inhibited by 5 or 10 mM iodacetamide | Pecten maximus | |
p-chloromercuribenzoate | - |
Pecten maximus |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.0198 | - |
NADH | wild type recombinant enzyme, in 50 mM triethanolamine hydrochloride buffer pH 7.0, at 25°C | Pecten maximus | |
0.0214 | - |
NADH | mutant enzyme D329A, in 50 mM triethanolamine hydrochloride buffer pH 7.0, at 25°C | Pecten maximus | |
0.0389 | - |
NADH | mutant enzyme C148S, in 50 mM triethanolamine hydrochloride buffer pH 7.0, at 25°C | Pecten maximus | |
0.04 | - |
NADH | mutant enzyme C148A, in 50 mM triethanolamine hydrochloride buffer pH 7.0, at 25°C | Pecten maximus | |
0.0649 | - |
NADH | mutant enzyme H212A, in 50 mM triethanolamine hydrochloride buffer pH 7.0, at 25°C | Pecten maximus | |
0.0904 | - |
NADH | mutant enzyme R324A, in 50 mM triethanolamine hydrochloride buffer pH 7.0, at 25°C | Pecten maximus | |
0.5 | - |
L-Arg | wild type recombinant enzyme, in 50 mM triethanolamine hydrochloride buffer pH 7.0, at 25°C | Pecten maximus | |
0.8 | - |
pyruvate | wild type recombinant enzyme, in 50 mM triethanolamine hydrochloride buffer pH 7.0, at 25°C | Pecten maximus | |
1.6 | - |
L-Arg | mutant enzyme C148A, in 50 mM triethanolamine hydrochloride buffer pH 7.0, at 25°C | Pecten maximus | |
1.7 | - |
L-Arg | mutant enzyme C148S, in 50 mM triethanolamine hydrochloride buffer pH 7.0, at 25°C | Pecten maximus | |
2.5 | - |
pyruvate | mutant enzyme C148A, in 50 mM triethanolamine hydrochloride buffer pH 7.0, at 25°C | Pecten maximus | |
2.6 | - |
pyruvate | mutant enzyme C148S, in 50 mM triethanolamine hydrochloride buffer pH 7.0, at 25°C | Pecten maximus | |
3.5 | - |
L-Arg | mutant enzyme H212A, in 50 mM triethanolamine hydrochloride buffer pH 7.0, at 25°C | Pecten maximus | |
9.8 | - |
L-Arg | mutant enzyme D329A, in 50 mM triethanolamine hydrochloride buffer pH 7.0, at 25°C | Pecten maximus | |
33 | - |
pyruvate | mutant enzyme D329A, in 50 mM triethanolamine hydrochloride buffer pH 7.0, at 25°C | Pecten maximus | |
59 | - |
pyruvate | mutant enzyme H212A, in 50 mM triethanolamine hydrochloride buffer pH 7.0, at 25°C | Pecten maximus | |
92 | - |
pyruvate | mutant enzyme R324A, in 50 mM triethanolamine hydrochloride buffer pH 7.0, at 25°C | Pecten maximus | |
159 | - |
L-Arg | mutant enzyme R324A, in 50 mM triethanolamine hydrochloride buffer pH 7.0, at 25°C | Pecten maximus |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
43000 | - |
His-tagged recombinant enzyme, SDS-PAGE | Pecten maximus |
43300 | - |
calculated from sequence of cDNA | Pecten maximus |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Pecten maximus | Q9BHM6 | - |
- |
Purification (Comment) | Organism |
---|---|
Ni-NTA affinity chromatography and Sephadex G-100 gel filtration | Pecten maximus |
Source Tissue | Comment | Organism | Textmining |
---|---|---|---|
muscle | - |
Pecten maximus | - |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
63 | - |
crude extract, at 18°C | Pecten maximus |
464 | - |
His-tagged recombinant enzyme, after purification, at 18°C | Pecten maximus |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
L-Arg + pyruvate + NADH | - |
Pecten maximus | N2-(D-1-carboxyethyl)-L-Arg + NAD+ + H2O | - |
? |
Synonyms | Comment | Organism |
---|---|---|
N2-(D-1-carboxyethyl)-L-arginine:NAD+-oxidoreductase | - |
Pecten maximus |
Octopine dehydrogenase | - |
Pecten maximus |
ODH | - |
Pecten maximus |
Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
2 | 8 | L-Arg | mutant enzyme D329A, in 50 mM triethanolamine hydrochloride buffer pH 7.0, at 25°C | Pecten maximus | |
5.1 | - |
L-Arg | mutant enzyme H212A, in 50 mM triethanolamine hydrochloride buffer pH 7.0, at 25°C | Pecten maximus | |
7.3 | - |
NADH | mutant enzyme H212A, in 50 mM triethanolamine hydrochloride buffer pH 7.0, at 25°C | Pecten maximus | |
9.3 | - |
NADH | mutant enzyme D329A, in 50 mM triethanolamine hydrochloride buffer pH 7.0, at 25°C | Pecten maximus | |
13 | - |
pyruvate | mutant enzyme H212A, in 50 mM triethanolamine hydrochloride buffer pH 7.0, at 25°C | Pecten maximus | |
15.4 | - |
pyruvate | mutant enzyme D329A, in 50 mM triethanolamine hydrochloride buffer pH 7.0, at 25°C | Pecten maximus | |
22.5 | - |
pyruvate | mutant enzyme R324A, in 50 mM triethanolamine hydrochloride buffer pH 7.0, at 25°C | Pecten maximus | |
25 | - |
NADH | mutant enzyme R324A, in 50 mM triethanolamine hydrochloride buffer pH 7.0, at 25°C | Pecten maximus | |
43 | - |
L-Arg | mutant enzyme R324A, in 50 mM triethanolamine hydrochloride buffer pH 7.0, at 25°C | Pecten maximus | |
410 | - |
NADH | mutant enzyme C148S, in 50 mM triethanolamine hydrochloride buffer pH 7.0, at 25°C | Pecten maximus | |
422 | - |
NADH | mutant enzyme C148A, in 50 mM triethanolamine hydrochloride buffer pH 7.0, at 25°C | Pecten maximus | |
444 | - |
L-Arg | mutant enzyme C148A, in 50 mM triethanolamine hydrochloride buffer pH 7.0, at 25°C | Pecten maximus | |
446 | - |
L-Arg | mutant enzyme C148S, in 50 mM triethanolamine hydrochloride buffer pH 7.0, at 25°C | Pecten maximus | |
499 | - |
pyruvate | mutant enzyme C148A, in 50 mM triethanolamine hydrochloride buffer pH 7.0, at 25°C | Pecten maximus | |
511 | - |
pyruvate | mutant enzyme C148S, in 50 mM triethanolamine hydrochloride buffer pH 7.0, at 25°C | Pecten maximus | |
640 | - |
L-Arg | wild type recombinant enzyme, in 50 mM triethanolamine hydrochloride buffer pH 7.0, at 25°C | Pecten maximus | |
652 | - |
NADH | wild type recombinant enzyme, in 50 mM triethanolamine hydrochloride buffer pH 7.0, at 25°C | Pecten maximus | |
775 | - |
pyruvate | wild type recombinant enzyme, in 50 mM triethanolamine hydrochloride buffer pH 7.0, at 25°C | Pecten maximus |
Cofactor | Comment | Organism | Structure |
---|---|---|---|
NADH | - |
Pecten maximus |
Organism | Comment | pI Value Maximum | pI Value |
---|---|---|---|
Pecten maximus | theoretical value | - |
5.35 |