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Literature summary for 1.5.1.15 extracted from

  • Christensen, K.E.; Mirza, I.A.; Berghuis, A.M.; Mackenzie, R.E.
    Magnesium and phosphate ions enable NAD binding to methylenetetrahydrofolate dehydrogenase-methenyltetrahydrofolate cyclohydrolase (2005), J. Biol. Chem., 280, 34316-34323.
    View publication on PubMed
Search for more literature for 1.5.1.15

Activating Compound

Activating Compound Comment Organism Structure
phosphate absolutely required, binds in close proximity to the 2’-hydroxyl of NAD through interactions with R166 and R198. Km-value of wild-type 0.412 mM Homo sapiens

Crystallization (Commentary)

Crystallization (Comment) Organism
construction of homology model Homo sapiens

Protein Variants

Protein Variants Comment Organism
D133A cosubstrate NAD+, 1.7% of wild-type activity, cosubstrate NADP+, 5.5% of wild-type activity Homo sapiens
D133E no enzymic activity Homo sapiens
D133N cosubstrate NAD+, 20.4% of wild-type activity, cosubstrate NADP+, 82.2% of wild-type activity Homo sapiens
D133S cosubstrate NAD+, 17.8% of wild-type activity, cosubstrate NADP+, 34.5% of wild-type activity Homo sapiens
D190A no enzymic activity Homo sapiens
D190E cosubstrate NAD+, 1% of wild-type activity Homo sapiens
D190N cosubstrate NAD+, 16% of wild-type activity Homo sapiens
D190S no enzymic activity Homo sapiens
R166A no enzymic activity Homo sapiens
R166K no enzymic activity Homo sapiens
R166S no enzymic activity Homo sapiens
R198A cosubstrate NAD+, 0.7% of wild-type activity, cosubstrate NADP+, 5.3% of wild-type activity Homo sapiens
R198K cosubstrate NAD+, 56% of wild-type activity, cosubstrate NADP+, 42% of wild-type activity Homo sapiens
R198S cosubstrate NAD+, 0.9% of wild-type activity, cosubstrate NADP+, 53% of wild-type activity Homo sapiens

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.202
-
 NAD+ wild-type, pH 7.3 Homo sapiens
0.255
-
 NADP+ mutant R198S, pH 7.3 Homo sapiens
0.352
-
 NADP+ wild-type, pH 7.3 Homo sapiens
0.503
-
 NADP+ mutant D133S, pH 7.3 Homo sapiens
0.58
-
 NADP+ mutant D133N, pH 7.3 Homo sapiens
0.689
-
 NADP+ mutant D190N, pH 7.3 Homo sapiens
0.918
-
 NAD+ mutant R198K, pH 7.3 Homo sapiens
1.99
-
 NAD+ mutant D133N, pH 7.3 Homo sapiens
2.12
-
 NADP+ mutant R198K, pH 7.3 Homo sapiens
2.17
-
 NAD+ mutant D133S, pH 7.3 Homo sapiens
3.88
-
 NAD+ mutant D190N, pH 7.3 Homo sapiens

Metals/Ions

Metals/Ions Comment Organism Structure
Mg2+ absolutely required Homo sapiens

Organism

Organism UniProt Comment Textmining
Homo sapiens P13995
-
-

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
0.024
-
 mutant D190E, cosubstrate NAD+, pH 7.3 Homo sapiens
0.156
-
 mutant R198A, cosubstrate NADP+, pH 7.3 Homo sapiens
0.16
-
 mutant D133A, cosubstrate NADP+, pH 7.3 Homo sapiens
0.17
-
 mutant R198A, cosubstrate NAD+, pH 7.3 Homo sapiens
0.207
-
 mutant R198S, cosubstrate NAD+, pH 7.3 Homo sapiens
0.392
-
 mutant D133A, cosubstrate NAD+, pH 7.3 Homo sapiens
1.01
-
 mutant D133S, cosubstrate NADP+, pH 7.3 Homo sapiens
1.24
-
 mutant R198K, cosubstrate NADP+, pH 7.3 Homo sapiens
1.54
-
 mutant R198S, cosubstrate NADP+, pH 7.3 Homo sapiens
2 2.5 wild-type, cosubstrate NAD+, pH 7.3 Homo sapiens
2.4
-
 mutant D133N, cosubstrate NADP+, pH 7.3 Homo sapiens
2.92
-
 wild-type, cosubstrate NADP+, pH 7.3 Homo sapiens
3.64
-
 mutant D190N, cosubstrate NAD+, pH 7.3 Homo sapiens
3.96
-
 mutant D190N, cosubstrate NADP+, pH 7.3 Homo sapiens
3.99
-
 mutant D133S, cosubstrate NAD+, pH 7.3 Homo sapiens
4.6
-
 mutant D133N, cosubstrate NAD+, pH 7.3 Homo sapiens
12.6
-
 mutant R198K, cosubstrate NAD+, pH 7.3 Homo sapiens

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
5,10-methylenetetrahydrofolate + NAD+
-
 Homo sapiens 5,10-methenyltetrahydrofolate + NADH
-
 ?
5,10-methylenetetrahydrofolate + NADP+ about 15% of the rate with NAD+ Homo sapiens 5,10-methenyltetrahydrofolate + NADPH + H+
-
 ?