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Literature summary for 1.5.1.17 extracted from

  • Fields, J.H.A.; Hochachka, P.W.
    Purification and properties of alanopine dehydrogenase from the adductur muscle of the oyster, Crassostrea gigas (mollusca, bivalvia) (1981), Eur. J. Biochem., 114, 615-621.
    View publication on PubMed

Inhibitors

Inhibitors Comment Organism Structure
2-oxoglutarate uncompetitive with respect to Ala and pyruvate Crassostrea gigas
ADP competitive with respect to NADH Crassostrea gigas
AMP competitive with respect to NADH Crassostrea gigas
ATP competitive with respect to NADH Crassostrea gigas
NAD+ competitive with respect to NADH Crassostrea gigas
oxamate
-
Crassostrea gigas
succinate
-
Crassostrea gigas

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.01
-
NADH
-
Crassostrea gigas
0.45
-
pyruvate
-
Crassostrea gigas
3.45
-
2-oxobutanoate
-
Crassostrea gigas
3.49
-
glyoxylate
-
Crassostrea gigas
6.48
-
Hydroxypyruvate
-
Crassostrea gigas
16
-
2-oxovalerate
-
Crassostrea gigas
96
-
Gly
-
Crassostrea gigas
98
-
L-Ala
-
Crassostrea gigas
150
-
L-2-aminobutyrate
-
Crassostrea gigas
680
-
Ser
-
Crassostrea gigas

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
46500
-
1 * 46500, SDS-PAGE Crassostrea gigas
47200
-
gel filtration Crassostrea gigas

Organism

Organism UniProt Comment Textmining
Crassostrea gigas
-
-
-

Purification (Commentary)

Purification (Comment) Organism
-
Crassostrea gigas

Source Tissue

Source Tissue Comment Organism Textmining
adductor
-
Crassostrea gigas
-

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
691
-
-
Crassostrea gigas

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
Gly + pyruvate + NADH
-
Crassostrea gigas Strombine + NAD+ + H2O
-
?
L-2-Aminobutyrate + pyruvate + NADH
-
Crassostrea gigas ?
-
?
L-2-aminobutyrate + pyruvate + NADH + H+
-
Crassostrea gigas ?
-
r
L-Ala + 2-oxobutanoate + NADH at 68% of the activity with pyruvate Crassostrea gigas ?
-
?
L-Ala + 2-oxopentanoate + NADH at 58% of the activity with pyruvate Crassostrea gigas ?
-
?
L-Ala + glyoxylate + NADH at 10.5% of the activity with pyruvate Crassostrea gigas ?
-
?
L-Ala + hydroxypyruvate + NADH at 3.3% of the activity with pyruvate Crassostrea gigas ?
-
?
L-Ala + pyruvate + NADH
-
Crassostrea gigas 2,2'-Iminodipropanoate + NAD+ + H2O
-
?
L-Ser + pyruvate + NADH
-
Crassostrea gigas ?
-
?

Subunits

Subunits Comment Organism
monomer 1 * 46500, SDS-PAGE Crassostrea gigas

Cofactor

Cofactor Comment Organism Structure
NAD+
-
Crassostrea gigas
NADH
-
Crassostrea gigas