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Literature summary for 1.5.1.2 extracted from
- Purification and characterization of rat lens pyrroline-5-carboxylate reductase (1986), Biochim. Biophys. Acta, 881, 72-78.
Inhibitors
| Inhibitors | Comment | Organism | Structure |
|---|---|---|---|
| ADP | - |
Rattus norvegicus |  |
| ATP | 1 mM | Rattus norvegicus | |
| Cd2+ | - |
Rattus norvegicus | |
| Cu2+ | - |
Rattus norvegicus | |
| Hg2+ | - |
Rattus norvegicus | |
| NADP+ | 1 mM | Rattus norvegicus | |
| p-chloromercuribenzoate | - |
Rattus norvegicus | |
| proline | 5 mM | Rattus norvegicus | |
KM Value [mM]
| KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 0.006 | - |
NADPH | - |
Rattus norvegicus | |
| 0.022 | - |
1-pyrroline-5-carboxylate | cosubstrate NADPH | Rattus norvegicus | |
| 0.051 | - |
DL-pyrroline-5-carboxylate | cosubstrate NADPH | Rattus norvegicus | |
| 0.21 | - |
1-pyrroline-5-carboxylate | cosubstrate NADH | Rattus norvegicus | |
| 0.37 | - |
NADH | - |
Rattus norvegicus | |
| 0.62 | - |
DL-pyrroline-5-carboxylate | cosubstrate NADH | Rattus norvegicus | |
Molecular Weight [Da]
| Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|
| 30000 | - |
SDS-PAGE | Rattus norvegicus |
| 240000 | - |
gel filtration | Rattus norvegicus |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Rattus norvegicus | - |
- |
- |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
- |
Rattus norvegicus |
Source Tissue
| Source Tissue | Comment | Organism | Textmining |
|---|---|---|---|
| lens | - |
Rattus norvegicus | - |
Specific Activity [micromol/min/mg]
| Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
|---|---|---|---|
| 720 | - |
- |
Rattus norvegicus |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| 1-pyrroline-5-carboxylate + NADH + H+ | - |
Rattus norvegicus | L-proline + NAD+ | - |
r | |
| L-Pro + NAD+ | - |
Rattus norvegicus | 1-pyrroline-5-carboxylate + NADH | - |
? | |
Subunits
| Subunits | Comment | Organism |
|---|---|---|
| octamer | 8 * 30000, SDS-PAGE | Rattus norvegicus |
| octamer | 8 * 30000 SDS-PAGE | Rattus norvegicus |
Temperature Stability [°C]
| Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 22 | - |
96 h stable | Rattus norvegicus |
pH Optimum
| pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|
| 6.5 | 7.1 | - |
Rattus norvegicus |
Cofactor
| Cofactor | Comment | Organism | Structure |
|---|---|---|---|
| NADPH | preferentially | Rattus norvegicus | |
Ki Value [mM]
| Ki Value [mM] | Ki Value maximum [mM] | Inhibitor | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 0.0037 | - |
NADP+ | against NADH | Rattus norvegicus | |
| 0.12 | - |
ATP | against DL-pyrroline-5-carboxylate | Rattus norvegicus | |
| 0.2 | - |
ATP | against NADH | Rattus norvegicus | |
| 0.32 | - |
ATP | against NADPH | Rattus norvegicus | |
| 0.38 | - |
NADP+ | against NADPH | Rattus norvegicus | |
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