Inhibitors | Comment | Organism | Structure |
---|---|---|---|
NADH | above 0.16 mM | Escherichia coli | |
NADP+ | - |
Escherichia coli | |
NADPH | above 0.13 mM | Escherichia coli | |
p-chloromercuribenzoate | complete inhibition at 0.125 mM, can be prevented by addition of dithiothreitol | Escherichia coli | |
proline | - |
Escherichia coli |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.14 | - |
1-pyrroline-5-carboxylate | with NADH | Escherichia coli | |
0.15 | - |
1-pyrroline-5-carboxylate | with NADPH | Escherichia coli | |
0.23 | - |
NADH | NADH | Escherichia coli |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
320000 | - |
gel filtration | Escherichia coli |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Escherichia coli | - |
K-12 | - |
Purification (Comment) | Organism |
---|---|
partial | Escherichia coli |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
41.25 | - |
- |
Escherichia coli |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
1-pyrroline-5-carboxylate + NAD(P)H + H+ | specific for L-isomer | Escherichia coli | L-proline + NAD(P)+ | - |
ir | |
1-pyrroline-5-carboxylate + NADH + H+ | - |
Escherichia coli | L-proline + NAD+ | - |
r | |
1-pyrroline-5-carboxylate + NADPH + H+ | - |
Escherichia coli | L-proline + NADP+ | - |
r |
Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
---|---|---|---|
4 | 40 | - |
Escherichia coli |
60 | - |
inactivation above | Escherichia coli |
67 | - |
50% inactivation after 5 min | Escherichia coli |
Cofactor | Comment | Organism | Structure |
---|---|---|---|
NADH | preferentially | Escherichia coli | |
NADPH | preferentially | Escherichia coli |