Literature summary for 1.5.1.2 extracted from

Meng, Z.; Lou, Z.; Liu, Z.; Hui, D.; Bartlam, M.; Rao, Z.
Purification, characterization, and crystallization of human pyrroline-5-carboxylate reductase (2006), Protein Expr. Purif., 49, 83-87.

Search for more literature for 1.5.1.2

Cloned(Commentary)

Cloned (Comment)	Organism
into a pET28a+ vector coding for a N-terminal His-tag for expression in Escherichia coli BL21DE3	Homo sapiens

Crystallization (Commentary)

Crystallization (Comment)	Organism
the protein is crystallized by the hanging-drop vapor-diffusion method at 37°C and diffraction data are obtained to a resolution of 2.8 A	Homo sapiens

Inhibitors

Inhibitors	Comment	Organism	Structure
proline	0.1 mM	Homo sapiens

KM Value [mM]

KM Value [mM]	KM Value Maximum [mM]	Substrate	Comment	Organism	Structure
0.141	-	3,4-dehydro-L-proline	-	Homo sapiens

Metals/Ions

Metals/Ions	Comment	Organism	Structure
Mg2+	-	Homo sapiens

Molecular Weight [Da]

Molecular Weight [Da]	Molecular Weight Maximum [Da]	Comment	Organism
370000	-	decamer, estimated by gel filtration and SDS-PAGE	Homo sapiens

Natural Substrates/ Products (Substrates)

Natural Substrates	Organism	Comment (Nat. Sub.)	Natural Products	Comment (Nat. Pro.)	Rev.	Reac.
1-pyrroline-5-carboxylate + NADPH + H+	Homo sapiens	-	L-proline + NADP+	-	r

Organism

Organism	UniProt	Comment	Textmining
Homo sapiens	P32322	-	-

Purification (Commentary)

Purification (Comment)	Organism
usung a Ni-NTA agarose, a Superdex-200 10/300 and a Resource Q column	Homo sapiens

Source Tissue

Source Tissue	Comment	Organism	Textmining
hepatoma cell line	full-length sequence is amplified from a hepatoma cell line cDNA library	Homo sapiens	-

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg]	Specific Activity Maximum [µmol/min/mg]	Comment	Organism
1.6	-	purification step: soluble cell fraction	Homo sapiens
14	-	purification step: Ni-NTA agarose	Homo sapiens
14	-	purification step: Resource Q	Homo sapiens
21	-	purification step: ammonium sulfate precipitation	Homo sapiens
32	-	purification step: Superdex 200	Homo sapiens

Storage Stability

Storage Stability	Organism
-80°C, 20 mM Caps, pH 9.4, 0.5 M NaCl, 6 months	Homo sapiens

Substrates and Products (Substrate)

Substrates	Comment Substrates	Organism	Products	Comment (Products)	Rev.	Reac.
1-pyrroline-5-carboxylate + NADPH + H+	-	Homo sapiens	L-proline + NADP+	-	r
3,4-dehydro-L-proline + NAD+	activity assay	Homo sapiens	?	-	?

Subunits

Subunits	Comment	Organism
decamer	-	Homo sapiens

Synonyms

Synonyms	Comment	Organism
P5CR	-	Homo sapiens
pyrroline-5-carboxylate reductase	-	Homo sapiens

Temperature Optimum [°C]

Temperature Optimum [°C]	Temperature Optimum Maximum [°C]	Comment	Organism
25	-	activity assay is performed at room temperature	Homo sapiens

Temperature Stability [°C]

Temperature Stability Minimum [°C]	Temperature Stability Maximum [°C]	Comment	Organism
90	-	irreversible loss of activity at 90°C for 5 min	Homo sapiens

Turnover Number [1/s]

Turnover Number Minimum [1/s]	Turnover Number Maximum [1/s]	Substrate	Comment	Organism	Structure
10	-	3,4-dehydro-L-proline	-	Homo sapiens

pH Optimum

pH Optimum Minimum	pH Optimum Maximum	Comment	Organism
9	-	activity assay	Homo sapiens

Cofactor

Cofactor	Comment	Organism	Structure
NADH	-	Homo sapiens
NADPH	-	Homo sapiens

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Release 2023.1 (January 2023)