Literature summary for 1.5.1.2 extracted from

Marchese, L.; Olavarria, K.; Mantilla, B.S.; Avila, C.C.; Souza, R.O.O.; Damasceno, F.S.; Elias, M.C.; Silber, A.M.
Trypanosoma cruzi synthesizes proline via a delta1-pyrroline-5-carboxylate reductase whose activity is fine-tuned by NADPH cytosolic pools (2020), Biochem. J., 477, 1827-1845 .

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Inhibitors

Inhibitors	Comment	Organism	Structure
NADPH	substrate inhibition	Trypanosoma cruzi

KM Value [mM]

KM Value [mM]	KM Value Maximum [mM]	Substrate	Comment	Organism	Structure
0.0128	-	NADPH	pH 7, 28°C	Trypanosoma cruzi
0.0277	-	1-pyrroline-5-carboxylate	pH 7, 28°C	Trypanosoma cruzi

Localization

Localization	Comment	Organism	GeneOntology No.	Textmining
cytosol	-	Trypanosoma cruzi	5829	-

Organism

Organism	UniProt	Comment	Textmining
Trypanosoma cruzi	Q4DH60	-	-
Trypanosoma cruzi CL Brener	Q4DH60	-	-

Substrates and Products (Substrate)

Substrates	Comment Substrates	Organism	Products	Comment (Products)	Rev.	Reac.
1-pyrroline-5-carboxylate + NADPH + H+	-	Trypanosoma cruzi	L-proline + NADP+	-	?
1-pyrroline-5-carboxylate + NADPH + H+	-	Trypanosoma cruzi CL Brener	L-proline + NADP+	-	?

Synonyms

Synonyms	Comment	Organism
P5CR	-	Trypanosoma cruzi
TcCLB.509207.90	-	Trypanosoma cruzi

Turnover Number [1/s]

Turnover Number Minimum [1/s]	Turnover Number Maximum [1/s]	Substrate	Comment	Organism	Structure
1	-	1-pyrroline-5-carboxylate	pH 7, 28°C	Trypanosoma cruzi
1.99	-	NADPH	pH 7, 28°C	Trypanosoma cruzi

IC50 Value

IC50 Value	IC50 Value Maximum	Comment	Organism	Inhibitor	Structure
0.045	-	pH 7, 28°C	Trypanosoma cruzi	NADPH

General Information

General Information	Comment	Organism
physiological function	Trypanosoma cruzi biosynthesizes proline from 1-pyrroline-5-carboxylate, which is produced exclusively from glutamate. P5CR shows a higher expression in the insect-resident form of the parasite. The model that best explains the obtained data includes a non-competitive substrate inhibition mechanism	Trypanosoma cruzi

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Release 2023.1 (January 2023)