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Literature summary for 1.5.1.20 extracted from

  • Zhou, J.; Kang, S.S.; Wong, P.W.K.; Fournier, B.; Rozen, R.
    Purification and characterization of methylenetetrahydrofolate reductase from human cadaver liver [published erratum appears in Biochem Med Metab Biol 1990 Oct;44(2):200] (1990), Biochem. Med. Metab. Biol., 43, 234-242.
    View publication on PubMed

General Stability

General Stability Organism
extremely sensitive to proteolysis Homo sapiens

Inhibitors

Inhibitors Comment Organism Structure
S-adenosylmethionine inhibition of the enzyme in crude extracts from fresh liver biopsies, but not after purification from cadaver liver Homo sapiens
tetrahydrofolate
-
Homo sapiens

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.012
-
5,10-methylenetetrahydrofolate NADPH-5,10-methylenetetrahydrofolate-oxidoreductase reaction Homo sapiens
0.017
-
NADPH NADPH-menadione oxidoreductase reaction Homo sapiens
0.038
-
5-methyltetrahydrofolate 5,10-methylenetetrahydrofolate-oxidoreductase reaction Homo sapiens

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
75000
-
SDS-PAGE Homo sapiens
150000
-
gel filtration Homo sapiens

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
5,10-methylenetetrahydrofolate + NADPH Homo sapiens first step in biosynthesis of methyl groups ?
-
?

Organism

Organism UniProt Comment Textmining
Homo sapiens
-
-
-

Purification (Commentary)

Purification (Comment) Organism
homogeneity Homo sapiens

Source Tissue

Source Tissue Comment Organism Textmining
liver
-
Homo sapiens
-

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
0.367
-
-
Homo sapiens

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
5,10-methylenetetrahydrofolate + NADPH
-
Homo sapiens 5-methyltetrahydrofolate + NADP+
-
?
5,10-methylenetetrahydrofolate + NADPH first step in biosynthesis of methyl groups Homo sapiens ?
-
?
5-methyltetrahydrofolate + oxidized menadione
-
Homo sapiens 5,10-methylenetetrahydrofolate + reduced menadione
-
?
NADPH + H+ + menadione
-
Homo sapiens NADP+ + menadiol
-
?

Subunits

Subunits Comment Organism
dimer 2 * 75000, SDS-PAGE and inhibition studies Homo sapiens

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
37
-
-
Homo sapiens

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
6.5
-
NADPH-tetrahydrofolate oxidoreductase activity Homo sapiens
6.6
-
methyltetrahydrofolate-menadione oxidoreductase activity Homo sapiens
7.2
-
NADPH-menadione oxidoreductase activity Homo sapiens

Cofactor

Cofactor Comment Organism Structure
FAD required Homo sapiens