Cloned (Comment) | Organism |
---|---|
overexpression in strain BL21(DE3) | Escherichia coli |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
66000 | - |
dimeric enzyme, gel filtration | Escherichia coli |
133000 | - |
tetrameric enzyme, gel filtration | Escherichia coli |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Escherichia coli | - |
- |
- |
Purification (Comment) | Organism |
---|---|
recombinant enzyme from strain BL21(DE3) | Escherichia coli |
Renatured (Comment) | Organism |
---|---|
dissociated enzyme after treatment with 1 M urea, no refolding and renaturation is possible after treatment with 1.2 M NaCl | Escherichia coli |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
5-methyltetrahydrofolate + NADP+ | - |
Escherichia coli | 5,10-methylenetetrahydrofolate + NADPH | - |
? |
Subunits | Comment | Organism |
---|---|---|
dimer | smallest functional unit of the enzyme | Escherichia coli |
More | alterations in the hydrophobic interactions by 1 M urea lead to dissociation of the native tetramer, resulting in stabilization of enzymatically active holoenzyme dimers, at 3 M urea followed by unfolding of the dimers to denatured monomers along with dissociation of FAD from the enzyme subunits, alterations of the electrostatic interactions by 1.2 M NaCl lead to dissociation of the enzyme into inactive, partially denatured dimers | Escherichia coli |
tetramer | composed of 2 active dimers | Escherichia coli |
Synonyms | Comment | Organism |
---|---|---|
5,10-methylenetetrahydrofolate reductase (FADH2) | - |
Escherichia coli |
5-methylenetetrahydrofolate:NADP+ oxidoreductase | - |
Escherichia coli |
5-methyltetrahydrofolate:(acceptor) oxidoreductase | - |
Escherichia coli |
methylenetetrahydrofolate reductase | - |
Escherichia coli |
methylenetetrahydrofolate reductase (NADPH) | - |
Escherichia coli |
More | EC 1.7.99.5 included with EC 1.5.1.20 | Escherichia coli |
MTHFR | - |
Escherichia coli |
Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
---|---|---|---|
51 | - |
dimeric enzyme, 50% inactivatin at pH 7.2 | Escherichia coli |
58 | - |
tetrameric enzyme, 50% inactivatin at pH 7.2 | Escherichia coli |
Cofactor | Comment | Organism | Structure |
---|---|---|---|
FAD | enzyme-bound | Escherichia coli | |
NADPH | - |
Escherichia coli |