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Literature summary for 1.5.1.21 extracted from
- DELTA1-Piperideine-2-carboxylate reductase of Pseudomonas putida (1982), J. Bacteriol., 149, 864-871.
Activating Compound
| Activating Compound | Comment | Organism | Structure |
|---|---|---|---|
| dithiothreitol | slight activation at 1 mM | Pseudomonas putida |  |
| EDTA | slight activation at 1 mM | Pseudomonas putida | |
| Sodium diphosphate | slight activation at 1 mM | Pseudomonas putida | |
General Stability
| General Stability | Organism |
|---|---|
| dithiothreitol, 1 mM, protects during storage | Pseudomonas putida |
| EDTA, 1 mM, protects during storage and against heat inactivation at 50°C | Pseudomonas putida |
| NADP+, no protection during storage | Pseudomonas putida |
| NADPH, protection during storage | Pseudomonas putida |
| sodium diphosphate, 1 mM, protects during storage | Pseudomonas putida |
| very unstable during purification | Pseudomonas putida |
Inhibitors
| Inhibitors | Comment | Organism | Structure |
|---|---|---|---|
| Ca2+ | slight inhibition | Pseudomonas putida | |
| Co2+ | almost total inhibition at 0.1 mM | Pseudomonas putida | |
| Hg2+ | almost total inhibition at 0.1 mM | Pseudomonas putida | |
| KCN | slight inhibition | Pseudomonas putida | |
| Mg2+ | slight inhibition | Pseudomonas putida | |
| Mn2+ | almost total inhibition at 0.1 mM | Pseudomonas putida | |
| additional information | no effect: 0.1 mM L-cysteine, 0.1 mM iodoacetate | Pseudomonas putida | |
| NADPH | substrate inhibition above 0.15 mM | Pseudomonas putida | |
| NaF | - |
Pseudomonas putida | |
| p-chloromercuribenzoate | almost total inhibition at 0.1 mM | Pseudomonas putida | |
| Zn2+ | almost total inhibition at 0.1 mM | Pseudomonas putida | |
KM Value [mM]
| KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 0.13 | - |
NADPH | - |
Pseudomonas putida | |
| 0.23 | - |
DELTA1-piperideine-2-carboxylate | - |
Pseudomonas putida | |
| 0.28 | - |
diglutamate | - |
Pseudomonas putida | |
| 0.4 | - |
DELTA1-Pyrroline-5-carboxylate | - |
Pseudomonas putida | |
Molecular Weight [Da]
| Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|
| 200000 | - |
gel filtration | Pseudomonas putida |
Natural Substrates/ Products (Substrates)
| Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| DELTA1-piperideine-2-carboxylate + NADPH | Pseudomonas putida | D-lysine metabolism | L-pipecolate + NADP+ | - |
? | |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Pseudomonas putida | - |
- |
- |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
- |
Pseudomonas putida |
Specific Activity [micromol/min/mg]
| Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
|---|---|---|---|
| 14.3 | - |
- |
Pseudomonas putida |
Storage Stability
| Storage Stability | Organism |
|---|---|
| 4°C, total loss of activity of the partially purified enzyme after 8 h, loss of activity can be prevented by adding low concentrations of EDTA, sodium diphosphate, dithiothreitol or L-pipecolic acid | Pseudomonas putida |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| DELTA1-piperideine-2-carboxylate + NADPH | - |
Pseudomonas putida | L-pipecolate + NADP+ | - |
ir | |
| DELTA1-piperideine-2-carboxylate + NADPH | D-lysine metabolism | Pseudomonas putida | L-pipecolate + NADP+ | - |
? | |
| DELTA1-pyrroline-5-carboxylate + NADPH + H+ | very little activity | Pseudomonas putida | ? | - |
r | |
| diglutamate + NADPH + H+ | very little activity | Pseudomonas putida | ? | - |
r | |
| additional information | poor substrates: DELTA1-pyrroline-5-carboxylate, pyroglutamate | Pseudomonas putida | ? | - |
? | |
Temperature Optimum [°C]
| Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 30 | - |
assay at | Pseudomonas putida |
pH Optimum
| pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|
| 8 | 8.3 | - |
Pseudomonas putida |
Cofactor
| Cofactor | Comment | Organism | Structure |
|---|---|---|---|
| NADH | poor coenzyme | Pseudomonas putida | |
| NADPH | - |
Pseudomonas putida | |
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