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Literature summary for 1.5.1.21 extracted from

  • Goto, M.; Muramatsu, H.; Mihara, H.; Kurihara, T.; Esaki, N.; Omi, R.; Miyahara, I.; Hirotsu, K.
    Crystal structures of DELTA1-piperideine-2-carboxylate/DELTA1-pyrroline-2-carboxylate reductase belonging to a new family of NAD(P)H-dependent oxidoreductases: conformational change, substrate recognition, and stereochemistry of the reaction (2005), J. Biol. Chem., 280, 40875-40884.
    View publication on PubMed
Search for more literature for 1.5.1.21

Crystallization (Commentary)

Crystallization (Comment) Organism
unliganded form, in complex with NADPH, and with NADPH and pyrrole-2-carboxylate. subunit consists of domain I, NADPH-binding domain II, and domain III. Identification of catalytic Asp-Ser-His triad Pseudomonas syringae

Inhibitors

Inhibitors Comment Organism Structure
2-picolinate
-
 Pseudomonas syringae
pyrrole-2-carboxylate
-
 Pseudomonas syringae

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
36000
-
 2 * 36000, SDS-PAGE, crystallization data Pseudomonas syringae

Organism

Organism UniProt Comment Textmining
Pseudomonas syringae Q4U331
-
-

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
140
-
 substrate pyruvate, 30°C, pH 10.0 Pseudomonas syringae
150
-
 substrate DELTA1-piperideine-2-carboxylate, 30°C, pH 10.0 Pseudomonas syringae
390
-
 substrate DELTA1-pyrroline-2-carboxylate, 30°C, pH 10.0 Pseudomonas syringae

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
alpha-ketobutanoate + methylamine + NADPH 10% of the rate with pyruvate Pseudomonas syringae N-methyl-2-aminobutanoate + NADP+ + H2O
-
 ?
alpha-ketohexanoate + methylamine + NADPH 23% of the rate with pyruvate Pseudomonas syringae N-methyl-2-aminohexanoate + NADP+ + H2O
-
 ?
DELTA1-piperideine-2-carboxylate + NADPH
-
 Pseudomonas syringae L-pipecolate + NADP+
-
 ?
DELTA1-pyrroline-2-carboxylate + NADPH
-
 Pseudomonas syringae L-proline + NADP+
-
 ?
fluoropyruvate + methylamine + NADPH 14% of the rate with pyruvate Pseudomonas syringae N-methylfluoroalanine + NADP+ + H2O
-
 ?
additional information no substrate: alpha-ketovalerate, ammonia Pseudomonas syringae ?
-
 ?
phenylpyruvate + methylamine + NADPH 6.3% of the rate with pyruvate Pseudomonas syringae N-methylphenylalanine + NADP+ + H2O
-
 ?
pyruvate + methylamine
-
 Pseudomonas syringae N-methylalanine
-
 ?

Subunits

Subunits Comment Organism
dimer 2 * 36000, SDS-PAGE, crystallization data Pseudomonas syringae

Synonyms

Synonyms Comment Organism
DpkA
-
 Pseudomonas syringae

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
30 45
-
 Pseudomonas syringae

Temperature Stability [°C]

Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
-
 35 stable for at least 30 min, pH 7.0 Pseudomonas syringae

Cofactor

Cofactor Comment Organism Structure
NADH specific activity with NADPH is 88fold higher than with NADH. Preferrence of NADPH over NADH is explained by cofactor binding site architecture Pseudomonas syringae
NADPH specific activity with NADPH is 88fold higher than with NADH. Preferrence of NADPH over NADH is explained by cofactor binding site architecture Pseudomonas syringae