Inhibitors | Comment | Organism | Structure |
---|---|---|---|
5,5'-dithiobis(2-nitrobenzoate) | sulfhydryl reagents restore activity | Haliotis discus hannai | |
Cu2+ | - |
Haliotis discus hannai | |
Fe2+ | - |
Haliotis discus hannai | |
Fluorescein mercuric acetate | sulfhydryl reagents restore activity | Haliotis discus hannai | |
Hg2+ | - |
Haliotis discus hannai | |
NAD+ | - |
Haliotis discus hannai | |
pyruvate | substrate inhibition above 8 mM | Haliotis discus hannai | |
tauropine | - |
Haliotis discus hannai | |
Zn2+ | - |
Haliotis discus hannai |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.071 | - |
NADH | - |
Haliotis discus hannai | |
3.6 | - |
2-oxovalerate | pyruvate | Haliotis discus hannai | |
44.4 | - |
taurine | - |
Haliotis discus hannai |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
42000 | - |
gel filtration | Haliotis discus hannai |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
additional information | Haliotis discus hannai | the enzyme plays an important physiological role in energy production during shell fixation activity in the columnella muscle | ? | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Haliotis discus hannai | - |
- |
- |
Purification (Comment) | Organism |
---|---|
- |
Haliotis discus hannai |
Source Tissue | Comment | Organism | Textmining |
---|---|---|---|
columella muscle | upper part | Haliotis discus hannai | - |
foot muscle | - |
Haliotis discus hannai | - |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
additional information | - |
- |
Haliotis discus hannai |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
Ala + pyruvate + NADH | about 2% of the activity with taurine | Haliotis discus hannai | ? | - |
? | |
additional information | the enzyme plays an important physiological role in energy production during shell fixation activity in the columnella muscle | Haliotis discus hannai | ? | - |
? | |
Taurine + 2-oxopentanoate + NADH | - |
Haliotis discus hannai | ? | - |
? | |
Taurine + pyruvate + NADH | r | Haliotis discus hannai | Tauropine + NAD+ + H2O | - |
? | |
Taurine + pyruvate + NADH | no activity with NADPH | Haliotis discus hannai | Tauropine + NAD+ + H2O | - |
? |
Subunits | Comment | Organism |
---|---|---|
monomer | 1 * 42000, SDS-PAGE | Haliotis discus hannai |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
30 | - |
- |
Haliotis discus hannai |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
7.2 | - |
tauropine formation | Haliotis discus hannai |
10 | - |
tauropine oxidation | Haliotis discus hannai |
Cofactor | Comment | Organism | Structure |
---|---|---|---|
NADH | no activity with NADPH | Haliotis discus hannai |