Literature summary for 1.5.1.23 extracted from

Plese, B.; Grebenjuk, V.A.; Schroeder, H.C.; Breter, H.J.; Mueller, I.M.; Mueller, W.E.
Cloning and expression of a tauropine dehydrogenase from the marine sponge Suberites domuncula (2008), Mar. Biol., 153, 1219-1232.

No PubMed abstract available

Cloned(Commentary)

Cloned (Comment)	Organism
cloning of the cDNA by PCR. DNA encoding TaDH is amplified by PCR from Suberites domuncula cDNA library, using the primers that are designed to amplify the complete open reading frame. Expression in Escherichia coli as His-tag fusion protein. Two allelic variants are identified, which are present in the different specimens either in a homozygotic or in a heterozygotic way (length polymorphism (453 bp and 468 bp long) is detected in the second intron and the beginning of third exon).	Suberites domuncula

Cloned (Comment)

Organism

cloning of the cDNA by PCR. DNA encoding TaDH is amplified by PCR from Suberites domuncula cDNA library, using the primers that are designed to amplify the complete open reading frame. Expression in Escherichia coli as His-tag fusion protein. Two allelic variants are identified, which are present in the different specimens either in a homozygotic or in a heterozygotic way (length polymorphism (453 bp and 468 bp long) is detected in the second intron and the beginning of third exon).

Suberites domuncula

Inhibitors

Inhibitors	Comment	Organism	Structure
additional information	level of expression of TaDH is controlled by oxygen: TaDH is expressed when the oxygen level is low, during anaerobic glycolysis	Suberites domuncula

Molecular Weight [Da]

Molecular Weight [Da]	Molecular Weight Maximum [Da]	Comment	Organism
36920	-	calculated from amino acid sequence	Suberites domuncula
36920	-	deduced from cDNA and determined by SDS polyacrylamide gele electrophoresis and Western blot analysis	Suberites domuncula

Natural Substrates/ Products (Substrates)

Natural Substrates	Organism	Comment (Nat. Sub.)	Natural Products	Comment (Nat. Pro.)	Rev.
additional information	Suberites domuncula	involvement of the sponge TaDH in the final step of the glycolytic pathway in the regeneration of NAD+ under anaerobic conditions	?	-	?
additional information	Suberites domuncula	opine production, opines are condensation products of an amino acid and a ketonic acid or a sugar	?	-	?
taurine + NADH + H+ + pyruvate	Suberites domuncula	taurine is involved in osmoregulation in marine animals	tauropine + NAD+ + H2O	-	?

Organism

Organism	UniProt	Comment	Textmining
Suberites domuncula	B1GT63	-	-
Suberites domuncula	B1GT63	demosponge	-

Purification (Commentary)

Purification (Comment)	Organism
Ni-NTA-column chromatography	Suberites domuncula
purification by metal-chelate affinity chromatography on a Ni-NTA-column	Suberites domuncula

Source Tissue

Source Tissue	Comment	Organism	Textmining
additional information	sponges are collected in Croatia and whole bodys are used for enzyme characterization, high levels of this enzyme in most of cell types	Suberites domuncula	-

Substrates and Products (Substrate)

Substrates	Comment Substrates	Organism	Products	Comment (Products)	Rev.
additional information	involvement of the sponge TaDH in the final step of the glycolytic pathway in the regeneration of NAD+ under anaerobic conditions	Suberites domuncula	?	-	?
additional information	opine production, opines are condensation products of an amino acid and a ketonic acid or a sugar	Suberites domuncula	?	-	?
taurine + NADH + H+ + pyruvate	taurine is involved in osmoregulation in marine animals	Suberites domuncula	tauropine + NAD+ + H2O	-	?
Taurine + pyruvate + NADH	-	Suberites domuncula	Tauropine + NAD+ + H2O	-	?

Synonyms

Synonyms	Comment	Organism
SD_TaDH	-	Suberites domuncula
TaDH	-	Suberites domuncula
tauropine dehydrogenase	belongs to the family of opine dehydrogenases, phylogenetic analyses indicate that TaDH is only distantly related to the opine dehydrogenases from marine invertebrates, high sequence similarity to bacterial ornithine cyclodeaminases	Suberites domuncula
tauropine [(carboxyethyl)-taurine/derived from sulfhydryl-amino acids] dehydrogenase	-	Suberites domuncula
tauropine [(carboxyethyl)-taurine/derived from sulfhydrylamino acids] dehydrogenase	-	Suberites domuncula

Cofactor

Cofactor	Comment	Organism	Structure
NAD(P)+	TaDH displays the GxGxxG/A motif known to be conserved among NAD(P)-dependent dehydrogenases	Suberites domuncula
NADH	-	Suberites domuncula

pI Value

Organism	Comment	pI Value Maximum	pI Value
Suberites domuncula	theoretical value	-	5.4
Suberites domuncula	calculated from amino acid sequence	-	5.44