Any feedback?
Please rate this page
(literature.php)
(0/150)

BRENDA support

Literature summary for 1.5.1.24 extracted from

  • Donkersloot, J.A.; Thompson, J.
    Cloning, expression, sequence analysis, and site-directed mutagenesis of the Tn5306-encoded N5-(carboxyethyl)ornithine synthase from Lactococcus lactis K1 (1995), J. Biol. Chem., 270, 12226-12234.
    View publication on PubMed
Search for more literature for 1.5.1.24

Application

Application Comment Organism
nutrition industrially important organism used widely as a starter in the dairy industry Lactococcus lactis
synthesis enzymatic biosyntheses of amino acids instead of chemical syntheses provide an attractive alternative to the former procedures since they are efficient and simple to perform. N,N-dialkylation does not occur and the enzyme-catalyzed reactions are both regio-and stereochemically specific Lactococcus lactis

Cloned(Commentary)

Cloned (Comment) Organism
ceo gene encoding N5-(carboxyethyl)ornithine synthase isolated from transposon Tn5306 of Lactococcus lactis K1 cloned and sequenced, expression in Escherichia coli CEO571 Lactococcus lactis

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
35320
-
 deduced from amino acid sequence Lactococcus lactis

Organism

Organism UniProt Comment Textmining
Lactococcus lactis
-
 subsp. lactis K1, formerly Streptococcus lactis K1
-
Lactococcus lactis
-
 formerly Streptococcus lactis K1-23
-
Lactococcus lactis K1-23
-
 formerly Streptococcus lactis K1-23
-

Purification (Commentary)

Purification (Comment) Organism
-
 Lactococcus lactis

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
L-ornithine + pyruvate + NADPH
-
 Lactococcus lactis N5-(L-1-carboxyethyl)-L-ornithine + NADP+ + H2O
-
 ir
L-ornithine + pyruvate + NADPH
-
 Lactococcus lactis K1-23 N5-(L-1-carboxyethyl)-L-ornithine + NADP+ + H2O
-
 ir
lysine + pyruvate + NADPH
-
 Lactococcus lactis N7-(1-carboxyethyl)lysine + NADP+ + H2O
-
 ir
lysine + pyruvate + NADPH
-
 Lactococcus lactis K1-23 N7-(1-carboxyethyl)lysine + NADP+ + H2O
-
 ir

Synonyms

Synonyms Comment Organism
N5-(CE) ornithine synthase
-
 Lactococcus lactis