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Literature summary for 1.5.1.3 extracted from

  • Thibault, V.; Koen, M.J.; Gready, J.E.
    Enzymic properties of a new mechanism-based substrate for dihydrofolate reductase (1989), Biochemistry, 28, 6042-6049.
No PubMed abstract available

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.03
-
8-Methylpterin pH 5.0 Gallus gallus
0.03
-
8-Methylpterin pH 5.0 Bos taurus
0.13
-
8-Methylpterin pH 5.0 Homo sapiens
0.2
-
8-Methylpterin pH 7.4 Gallus gallus
0.2
-
8-Methylpterin pH 7.4 Bos taurus
0.3
-
8-Methylpterin above pH 7.4 Homo sapiens

Organism

Organism UniProt Comment Textmining
Bos taurus
-
-
-
Gallus gallus
-
-
-
Homo sapiens
-
methotrexate-resistant WIL-2 lymphoblastoid cells
-

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
8-methylpterin + NADPH
-
Gallus gallus 8-methyl-7,8-dihydropterin + NADP+ subsequently reduced more slowly and incompletely to 8-methyl-5,6,7,8-tetrahydropterin ?
8-methylpterin + NADPH
-
Homo sapiens 8-methyl-7,8-dihydropterin + NADP+ subsequently reduced more slowly and incompletely to 8-methyl-5,6,7,8-tetrahydropterin ?
8-methylpterin + NADPH
-
Bos taurus 8-methyl-7,8-dihydropterin + NADP+ subsequently reduced more slowly and incompletely to 8-methyl-5,6,7,8-tetrahydropterin ?

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
5.8 6 8-methylpterin + NADPH Gallus gallus
5.8 6 8-methylpterin + NADPH Homo sapiens
5.8 6 8-methylpterin + NADPH Bos taurus