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Literature summary for 1.5.1.3 extracted from
- Isolation and characterization of dihydrofolate reductase from trimethoprim-susceptible and trimethoprim-resistant Pseudomonas cepacia (1989), Antimicrob. Agents Chemother., 33, 1247-1251.
Inhibitors
| Inhibitors | Comment | Organism | Structure |
|---|---|---|---|
| methotrexate | - |
Burkholderia cepacia |  |
| trimethoprim | - |
Burkholderia cepacia | |
KM Value [mM]
| KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 0.003 | - |
7,8-dihydrofolate | strain PC 174 | Burkholderia cepacia | |
| 0.004 | - |
7,8-dihydrofolate | strain PC 178 | Burkholderia cepacia | |
Molecular Weight [Da]
| Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|
| 23000 | 24000 | SDS-PAGE | Burkholderia cepacia |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Burkholderia cepacia | - |
trimethoprim-susceptible strain PC 174 and trimethoprim-resistant strain PC 178 | - |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
- |
Burkholderia cepacia |
Specific Activity [micromol/min/mg]
| Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
|---|---|---|---|
| 5.59 | - |
strain PC 174 | Burkholderia cepacia |
| 10.4 | - |
strain PC 178 | Burkholderia cepacia |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| 7,8-dihydrofolate + NADPH + H+ | - |
Burkholderia cepacia | 5,6,7,8-tetrahydrofolate + NADP+ | - |
r | |
Subunits
| Subunits | Comment | Organism |
|---|---|---|
| ? | ? * 23000-24000, SDS-PAGE | Burkholderia cepacia |
Temperature Optimum [°C]
| Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 30 | - |
assay at | Burkholderia cepacia |
Temperature Stability [°C]
| Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
|---|---|---|---|
| additional information | - |
heat stable | Burkholderia cepacia |
| 45 | - |
70% activity remaining after 10 min | Burkholderia cepacia |
Cofactor
| Cofactor | Comment | Organism | Structure |
|---|---|---|---|
| NADPH | - |
Burkholderia cepacia | |
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Release 2023.1 (January 2023)
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